Sublethal exposure to spinetoram impacts life history traits and dengue virus replication in Aedes aegypti  

作　　者：Luoluo Wang Zhichang Pang Qi Chen Zhentao Song Yi Lu Meng Yang Jia Huang Xiao-Qiang Yu Xiaoyun Wang 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Instute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China [2]State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen University,Guangzhou,China

出　　处：《Insect Science》2023年第2期486-500,共15页昆虫科学（英文版）

基　　金：funded by the National Natural Science Foundation of China(82002166);the fellowship of China Postdoctoral Science Foundation(2020M672676);the Guangdong Project of Basic and Applied Basic Research(20201910240000150);funded by the National Natural Science Foundation of China(81902093);Guangzhou Science and Technology Project(202002030100);Funding from State Key Laboratory of Biocontrol(Sun Yat-sen University)(2020SKLBC-KF01).

摘　　要：Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or nontarget species has long been ignored.Here,we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus(DENV)infection.We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females,apart from the extended immature development period.In terms of reproductive potential,although the F_(0) generation produced a similar number of eggs and offspring during the first gonotrophic cycle,the survival rates of the F_(1) generations were significantly lower as compared to the control group,suggesting transgenerational sublethal effects on the F_(1) generation.Notably,surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal exposure.Mechanistically,transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult Ae.aegypti.In Aag2 cells,significant accumulation of apoptosis,mitochondrial reactive oxygen species production,and DENV-2 replication by spinetoram exposure consistently support our conclusion.Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.

关 键 词：Aedes aegypti dengue virus SPINETORAM sublethal exposure SUSCEPTIBILITY 

分 类 号：S48[农业科学—农药学]