柚苷废水中乳酸菌的筛选及其发酵工艺优化  

Screening of Lactic acid bacteria in naringin wastewater and optimization of fermentation process

作　　者：付文娟 黎茵 朱英卓 黄园园 杨懿 陈淑芳 王庆 FU Wenjuan;LI Yin;ZHU Yingzhuo;HUANG Yuanyuan;YANG Yi;CHEN Shufang;WANG Qing(Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas,Meizhou,Guangdong Province 514015,China;Meizhou Biological Feed Engineering Technology Research Center,Meizhou,Guangdong Province 514015,China;College of Life Sciences,Jiaying University,Meizhou,Guangdong Province 514015,China)

机构地区：[1]广东省山区特色农业资源保护与精准利用重点实验室,广东梅州514015 [2]梅州市生物饲料工程技术研究中心,广东梅州514015 [3]嘉应学院生命科学学院,广东梅州514015

出　　处：《中国饲料》2023年第9期30-37,共8页China Feed

基　　金：2020年广东省科技专项基金项目(2020A0104001);梅州市应用型科技专项基金项目(2019B0201006)。

摘　　要：本研究从自然发酵的柚苷废水中筛选获得了1株产酸和增殖能力较强的乳酸菌柚1,利用形态学、生理生化和16S rDNA测序鉴定其为植物乳杆菌(Lactobacillus plantarum)。以柚1作为发酵菌种进行柚苷废水发酵工艺优化,通过单因素和正交试验对发酵培养基热处理工艺、培养基组分和发酵条件进行优化,结果表明:发酵培养基的最佳热处理工艺为65℃水浴5 min,发酵培养基的最佳配方为:柚苷废水+1%玉米粉+1%豆粕,最适发酵条件为:接种量1.5%,培养温度32℃,培养时间30 h,在此条件下发酵柚苷废水中的菌数可达9.72×10^(8)cfu/mL,总酸度为11.62 g/L,乳酸含量为6.36 g/L。通过发酵处理,为柚苷废水的饲料化利用提供了可能,并为动物养殖提供了一种富含益生菌、有机酸及柚果中水溶性提取物的潜在饲料添加剂产品。In this study,a strain named pomelo 1 with strong acid production and proliferation ability was screened from naturally fermented naringin wastewater,and was identified as Lactobacillus plantarum by morphological observation,physiological and biochemical tests and 16S rDNA sequencing.The selection and optimization of heat treatment process,medium constitutes as well as culture conditions was carried out using one-factor-at-a-time method combined with orthogonal experiment.As a result,the optimum heat treatment process was 65℃water bath heating for 5 min,the optimal levels for corn flour,soybean meal,inoculation amount,culture temperature and culture time were determined as 1%,1%,1.5%,32℃and 30 h,respectively.Confimatory experiments performed under this conditions yielded a maximam of bacteria counts 9.72×10^(8) cfu/mL,total acidity 11.62 g/L and lactica acid content 6.36 g/L.Fermentation treatment provide the possibility for the feed utilization of naringin wastewater,and provide a potential feed additive product rich in probiotics,organic acids and water-soluble extracts from pomelo fruit for animal breeding.

关 键 词：植物乳杆菌 柚苷废水 热处理工艺 发酵条件优化 

分 类 号：S816.3[农业科学—饲料科学]