楤木属及其近缘类群叶绿体基因组比较与系统发育分析  

Comparative chloroplast genomic and phylogenetic analysis of Aralia and related species

作　　者：王孟豪 冉航 刘艳艳 孙华越 曹亚男 王红卫[1] 李家美[2] Wang Meng-Hao;Ran Hang;Liu Yan-Yan;Sun Hua-Yue;Cao Ya-Nan;Wang Hong-Wei;Li Jia-Mei(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China;College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002 [2]河南农业大学生命科学学院,郑州450002

出　　处：《植物科学学报》2023年第2期149-158,共10页Plant Science Journal

基　　金：国家自然科学基金(31800179)。

摘　　要：分别对楤木属(Aralia)和羽叶参属(Pentapanax)各两个物种进行叶绿体基因组测序、组装和注释,并进行结构分析;结合NCBI下载的近缘类群叶绿体组序列,进行系统发育分析。结果显示,4个物种的叶绿体基因组均为环状四分体结构,长度为155 744~156 201 bp,GC含量为38.1%,均包含132个基因,其中蛋白质编码基因87个,rRNA基因8个,t RNA基因37个。边界分析发现IR(Inverted repeat)区均未发生收缩和扩张。SSR(Simple sequence repeat)序列数量在39~43个,多为单核苷酸和二核苷酸重复,位置多在非编码区。序列差异多出现在LSC(Large single copy)和SSC(Small single copy)区的非编码区。最大似然树揭示出两个高度支持的单系分支:第1分支包括羽叶参属、浓紫龙眼独活(Aralia atropurpurea Franch.)、食用土当归(Aralia cordata Thunb.)和东北土当归(Aralia continentalis Kitagawa);第2分支则均由楤木属物种组成。总体来说,楤木属和羽叶参属植物的叶绿体基因组序列比较保守;系统发育分析结果支持羽叶参属置入楤木属内部以及楤木属的各个种成单系。The chloroplast genomes of two species of Aralia and Pentapanax were sequenced,assembled,annotated,and structurally analyzed.Furthermore,combined with chloroplast genome sequences of related species downloaded from the NCBI database,phylogenetic analysis was conducted.The chloroplast genomes of the four species all exhibited a quadripartite circular structure with a length of 155744–156201 bp and GC content of 38.1%.Both genera contained 132 genes,including 87 protein-coding genes,eight ribosomal RNA(rRNA)genes,and 37 transfer RNA(tRNA)genes.Boundary analysis found that neither contraction nor expansion occurred in the inverted repeat regions.The number of simple sequence repeat(SSR)loci ranged from 39 to 43,most of which were mononucleotide and dinucleotide repeats,and mostly located in noncoding regions.Sequence differences mainly occurred in the non-coding regions of the large single copy(LSC)and small single copy(SSC)regions.The maximum-likelihood tree revealed two highly supported monophyletic branches:the first composed of Pentapanax,Aralia atropurpurea Franch.,Aralia cordata Thunb.,and Aralia continentalis Kitagawa and the second composed of other Aralia species.In general,the chloroplast genome sequences of Aralia and Pentapanax plants were relatively conserved.The phylogenetic results supported the placement of Pentapanax into the genus Aralia,with each Aralia species forming a monophyly.

关 键 词：楤木属 羽叶参属 叶绿体基因组 系统发育 

分 类 号：Q943.2[生物学—植物学]