微RNA-205与人食管癌放射治疗抵抗细胞株Eca-109R放射抗性的相关性  

Relationship between microRNA-205 and radiation resistance of human esophageal carcinoma radiation resistant cell line Eca-109R

作　　者：赵文玺 张晶晶 王鹏 徐广峰 吴德平 ZHAO Wenxi;ZHANG Jingjing;WANG Peng;XU Guangfeng;WU Deping(Clinical Laboratory,the 82 nd Hospital of Huai′an〔the Huai′an Medical Area of the Former Eastern Theater General Hospital〕,Huai′an 223001,Jiangsu Province,China;the Dingwei Medical Laboratory of Huai′an,Huai′an 223001,Jiangsu Province,China;Clinical Laboratory,Huai′an No.3 People′s Hospital,Huai′an 223001,Jiangsu Province,China)

机构地区：[1]淮安八十二医院〔原东部战区总医院淮安医疗区〕检验科,江苏淮安223001 [2]淮安鼎卫医学检验所,江苏淮安223001 [3]淮安市第三人民医院检验科,江苏淮安223001

出　　处：《新乡医学院学报》2023年第5期411-415,共5页Journal of Xinxiang Medical University

基　　金：淮安市自然科学基金资助项目(编号:HAB202133)。

摘　　要：目的探讨人食管癌放射治疗抵抗细胞株Eca-109R中微RNA(miR)-205的表达水平与放射抗性的相关性。方法取人食管鳞状细胞癌细胞株Eca-109,采用多次逐渐递增剂量的射线照射建立人食管癌放射治疗抵抗细胞株Eca-109R;另取Eca-109细胞,待细胞培养至融合度达到50%~70%时将miR-205 mimics转染入Eca-109细胞,形成Eca-109 miR-205过表达细胞。根据照射剂量将Eca-109细胞、Eca-109R细胞和Eca-109 miR-205过表达细胞分别分为0 Gy组、2 Gy组、4 Gy组、6 Gy组、8 Gy组、10 Gy组。用倒置显微镜观察Eca-109和Eca-109R细胞的形态,细胞计数试剂盒-8实验检测Eca-109细胞、Eca-109R细胞和Eca-109 miR-205过表达细胞的存活率;细胞克隆形成实验检测Eca-109细胞、Eca-109R细胞和Eca-109 miR-205过表达细胞的增殖情况;反转录聚合酶链式反应检测Eca-109细胞、Eca-109R细胞和Eca-109 miR-205过表达细胞中miR-205的表达水平。结果Eca-109细胞形态均一,大小一致,大多呈片状或梭状,折光性良好;Eca-109R细胞可见少量多角形细胞,伴有颗粒感,折光性差。在0 Gy组,Eca-109R细胞、Eca-109 miR-205过表达细胞与Eca-109细胞的存活率和细胞增殖能力比较差异无统计学意义(P>0.05)。在2、4、6 Gy组,Eca-109R细胞、Eca-109 miR-205过表达细胞的存活率和细胞增殖能力显著高于Eca-109细胞(P<0.05)。在8 Gy组,Eca-109R细胞的存活率和细胞增殖能力显著高于Eca-109细胞(P<0.05);Eca-109 miR-205过表达细胞与Eca-109细胞的存活率和细胞增殖能力比较差异无统计学意义(P>0.05)。在10 Gy组,Eca-109R细胞、Eca-109 miR-205过表达细胞与Eca-109细胞的存活率和细胞增殖能力比较差异无统计学意义(P>0.05)。在0 Gy组,Eca-109R细胞、Eca-109 miR-205过表达细胞中miR-205的相对表达量显著高于Eca-109细胞(P<0.05)。在4 Gy组,Eca-109R细胞中miR-205的相对表达量显著高于Eca-109细胞(P<0.05)。4 Gy组的Eca-109细胞中miR-205的相对表达量显著�Objective To investigate the relationship between the expression of microRNA(miR)-205 and radiation resistance in human esophageal carcinoma radiation resistant cell line Eca-109R.Methods Human esophageal carcinoma cell lines Eca-109 were taken and irradiated by multiple increasing doses of radiation to establish the human esophageal carcinoma radiation resistant cell line Eca-109R.Another Eca-109 cells were taken and cultured,when the fusion degree of Eca-109 cells reached 50%-70%,the miR-205 mimics were transfected into Eca-109 cells to form the Eca-109 miR-205 overexpression cells.The Eca-109 cells,Eca-109R cells and Eca-109 miR-205 overexpression cells were divided into 0 Gy group,2 Gy group,4 Gy group,6 Gy group,8 Gy group and 10 Gy group according to irradiation doses.The morphology of Eca-109 and Eca-109R cells was observed by inverted microscope;the viability of Eca-109 cells,Eca-109R cells and Eca-109 miR-205 overexpression cells was detected by cell counting kit-8 assay;the proliferation ability of Eca-109 cells,Eca-109R cells and Eca-109 miR-205 overexpression cells was detected by colony formation assay;the expression levels of miR-205 in Eca-109 cells,Eca-109R cells and Eca-109 miR-205 overexpression cells were detected by reverse transcription-polymerase chain reaction.Results Eca-109 cells had uniform morphology and consistent size,and were mostly sheet or spindle shape;Eca-109 cells had good refractive index.In the Eca-109R cells,there were small amounts of polygonal cells with a granular sensation and poor refractive index.In the 0 Gy group,there was no significant difference in the survival rates and proliferation ability between Eca-109R cells,Eca-109 miR-205 overexpression cells and Eca-109 cells(P>0.05).In the 2,4,6 Gy groups,the survival rates and proliferation ability of Eca-109R cells and Eca-109 miR-205 overexpression cells were significantly higher than those of Eca-109 cells(P<0.05).In the 8 Gy group,the survival rate and proliferation ability of Eca-109R cells were significantly higher

关 键 词：食管癌 Eca-109R细胞株 放射抗性 微RNA-205 

分 类 号：R735.1[医药卫生—肿瘤]