不同贮藏温度下白兰瓜蔗糖代谢途径中相关酶表达量分析  

Study of the Activities of Enzymes Related to the Sucrose Metabolism Pathway of Honeydew Melon under Different Storage Temperatures

作　　者：刘丹阳 鲁丁强 任瑞娟 刘玉姣 刘一璇 王新倩 庞广昌[1,2] LIU Dan-yang;LU Ding-qiang;REN Rui-juan;LIU Yu-jiao;LIU Yi-xuan;WANG Xin-qian;PANG Guang-chang(College of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;Tianjin Key Laboratory of Food Biotechnology,Tianjin 300134,China;Tianjin Institute for Food Safety Inspection Technology,Tianjin 300308,China)

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134 [2]天津市食品生物技术重点实验室,天津300134 [3]天津市食品安全检测技术研究院,天津300308

出　　处：《食品研究与开发》2023年第9期15-22,共8页Food Research and Development

基　　金：国家自然科学基金青年科学基金项目(31901782)。

摘　　要：为了解不同温度贮藏对白兰瓜蔗糖代谢途径中相关酶表达量的影响,在不同温度梯度下(0、4、8、10、15、20、25、30、35、40℃)贮藏24 h,测定代谢途径中的相关酶表达量。结果表明,低温贮藏抑制白兰瓜蔗糖代谢途径中相关酶表达量的升高,其中,0℃的贮藏条件抑制丙酮酸激酶、磷酸果糖激酶等酶表达量的升高,4℃贮藏抑制己糖激酶、磷酸丙糖异构酶等酶表达量升高,8、10℃贮藏抑制α-酮戊二酸脱氢酶、乳酸脱氢酶、磷酸丙糖异构酶、醛缩酶等酶表达量的升高,低温贮藏可以有效控制白兰瓜蔗糖代谢处于最低水平,维持果实品质,延长贮藏期。The aim of this study was to understand the effect of different storage temperatures on enzymes related to the sucrose metabolic pathway of honeydew melon.The activities of enzymes related to the metabolic pathway were measured after storage at different temperatures(0,4,8,10,15,20,25,30,35℃and 40℃)for 24 h.The results showed that low-temperature storage inhibited the increase in the activity of enzymes related to the sucrose metabolic pathway of honeydew melon.Storage at 0℃inhibited the increase in pyruvate kinase and phosphofructokinase activity,and storage at 4℃inhibited the increase in hexokinase activity.The activity of enzymes such as triose phosphate isomerase increased and storage at 8℃and 10℃inhibited the increase inα-ketoglutarate dehydrogenase,lactate dehydrogenase,triose phosphate isomerase,and aldolase activities.Under low temperature storage conditions,the sucrose metabolism of honeydew melon was effectively controlled,the fruit quality was maintained,and the storage period was prolonged.

关 键 词：白兰瓜 低温贮藏 酶表达量 蔗糖代谢 呼吸代谢 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]