刺山柑乙醇提取物乳膏对系统性硬皮病小鼠Notch1、DLL1和ADAM17表达的影响  

Effects of Capparis Spinosa Ethanolic Extract Cream on Expressions of Notch1,DLL1 and ADAM17 in Systemic Sclerosis Mice

作　　者：迪力努尔·艾尼 阿依提拉·麦麦提 康小龙[2,3] Dilinuer·Aini;Ayitila·Maimaiti;Kang Xiaolong(The Fourth College of Clinical Medicine,Xinjiang Medical University,Urumqi 830000,China;The Affiliated Traditional Chinese Medicine Hospital of Xingjiang Medcial University,Urumqi 830000,China;Xinjiang Key Laboratory of Processing and Research of Traditional Chinese Medicine,Urumqi 830000,China)

机构地区：[1]新疆医科大学第四临床医学院,乌鲁木齐830000 [2]新疆医科大学附属中医医院,乌鲁木齐830000 [3]新疆中药炮制研究重点实验室,乌鲁木齐830000

出　　处：《中国药事》2023年第4期420-425,共6页Chinese Pharmaceutical Affairs

基　　金：新疆维吾尔自治区自然科学基金(编号2022D01C557)。

摘　　要：目的:在系统性硬皮病(SSc)模型小鼠上研究刺山柑乙醇提取物乳膏对Notch通路中重要蛋白Notch1、DLL1和ADAM17的调控作用。方法:给予刺山柑乙醇提取物乳膏冶疗SSc模型小鼠(模型应用BALB/c小鼠皮下注射博莱霉素4 w建立)8 w。小鼠皮肤组织中Notch1 mRNA和蛋白的表达分别采用实时荧光逆转录PCR(qPCR)和免疫组化法测定,DLL1和ADAM17含量采用ELISA法测定。结果:过表达的Notch1 mRNA和蛋白可被450、900 mg·kg^(-1)刺山柑乙醇提取物乳膏抑制,DLL1和ADAM17可被900mg·kg^(-1)刺山柑乙醇提取物乳膏抑制。结论:SSc小鼠受损皮肤Notch通路中过表达的Notch1、DLL1和ADAM17可被刺山柑乙醇提取物乳膏抑制,提示其对SSc Notch通路的过度激活有一定抑制作用。Objective:To explore the regulation effects of capparis spinosa ethanolic extract cream on expressions of Notch1,DLL1 and ADAM17 in Notch pathway in Systemic Sclerosis(SSc)mice.Methods:SSc modelmice were treated with capparis spinosa ethanolic extract cream(the model was established by subcutaneous injection of bleomycin for 4 w in BALB/c mice)for 8 w.The mRNA expression of Notch1 and protein in mouse skin were detected by real-time reverse transcriptase PCR(qPCR)and immunohistochemical method,respectively.The contents of DLL1 and ADAM17 were measured by ELISA in skin tissue.Results:The mRNA and protein expression of Notch1 were inhibited by 450 and 900 mg·kg^(-1) capparis spinosa ethanolic extract cream and DLL1 and ADAM17 were inhibited by 900 mg·kg^(-1) capparis spinosa ethanolic extract cream.Conclusion:Capparis spinosa ethanolic extract cream could inhibit the Notch pathway in damaged skin of SSc mice by down-regulating the expressions of Notch1,DLL1 and ADAM17,suggesting that the SSc Notch pathway overactivation could be inhibited to some extent.

关 键 词：刺山柑乙醇提取物乳膏 系统性硬皮病 NOTCH1 DLL1 ADAM17 

分 类 号：R965.1[医药卫生—药理学]