反向离子导入对兔眼前房积血治疗的实验研究  

Evaluation of Treatment by Reverse Iontophoresis in a Rabbit Hyphema Model Introduction

作　　者：孙守彬[1] 杨洁 SUN Shou-bin;YANG Jie(Department of Ophthalmology,The Fourth Affiliated Hospital of Harbin Medical University,Harbin Heilongjiang,150001,China;Department of Otolaryngology,The Second Hospital of Heilongjiang Province,Harbin Heilongjiang,150000,China)

机构地区：[1]哈尔滨医科大学附属第四医院眼科,黑龙江哈尔滨150001 [2]黑龙江省第二医院耳鼻喉科,黑龙江哈尔滨150000

出　　处：《中华养生保健》2023年第10期1-4,共4页CHINESE HEALTH CARE

基　　金：黑龙江省卫生计生委科研课题(2018-004);哈尔滨医科大学附属第四医院优秀青年项目(HYDSYYXQN202021)。

摘　　要：目的利用反向离子导入经角膜提取兔眼前房内铁离子以治疗前房积血。方法24只白兔随机分为3组,每组8只。其中,Ⅰ组8只白兔不造模为空白对照组。16只白兔右眼制造前房积血模型,造模后再随机分成Ⅱ、Ⅲ两组,每组8只眼。Ⅱ组为模型对照组,给予0 mA电流强度反向离子导入治疗,观察前房积血的自然变化;Ⅲ组为治疗组,经角膜反向离子电渗技术治疗,每日给予0.4 mA电流强度经角膜反向离子导入技术治疗,15 min/次,持续治疗7 d。利用裂隙灯显微镜观察前房积血的吸收速度,利用电感耦合等离子体发射光谱仪测量各组房水中铁离子的浓度,利用组织学检查观察角膜的病理改变。结果房水中铁离子浓度模型对照组明显高于空白对照组和治疗组,差异有统计学意义(P<0.05)。裂隙灯显微镜观察治疗组前房积血的吸收速度明显优于对照组,组织学检查治疗组角膜炎细胞浸润及铁颗粒沉积明显优于模型组。结论反向离子导入可以使兔眼前房内的铁离子通过角膜排除体外,从而对前房积血治疗具有重要意义。Objective The purpose of this study was to treat hyphema by using reverse ion introductio to extract iron from anterior chamber of the rabbit cornea.Methods 24 rabbits were randomly divided into three groups,with 8 rabbits in each group.The 8 rabbits in group I were not modeled as the blank control group;The anterior chamber blood model was fabricated in the right eyes of 16 rabbits,and after modeling,they were randomly divided into two groupsⅡandⅢwith 8 eyes in each group.The groupⅡwas called model control group,which was given 0 ma current intensity reverse iontophoresis treatment to observe the natural changes of anterior chamber blood volume;The groupⅢwas treated by transcorneal reverse iontophoresis technique at a current intensity of 0.4 Ma once daily for 15 min for seven days.The absorption velocity of the anterior chamber blood was observed by slit lamp microscopy,the concentration of iron ions in the aqueous humor of each group was measured by inductively coupled plasma optical emission spectrometer,and the pathological changes of the cornea were observed by histological examination.Results The iron concentration in aqueous humor was greatly increased in the modeling group compared with control group,but it was greatly reduced by reverse iontophoresis treatment.Histological examination indicated that inflammatory cell infiltration and iron deposition in the RI treatment group were significantly less than the modeling group.Conclusions Reverse iontophoresis in cornea is a non-invasive and promising approach to extract iron from the anterior chamber to treat hyphema.

关 键 词：反向离子导入 前房积血 铁 

分 类 号：R779.1[医药卫生—眼科]