基于生物信息学分析miR-152在子痫前期发病机制中的作用  

Bioinformatics-based Analysis of The Role of miR-152 in The Pathogenesis of Pre-eclampsia

作　　者：杨倩文[1] 陶峰[1] 陈红波[1] Yang Qianwen;Tao Feng;Chen Hongbo(Anhui maternal and Child Health Hospital,Hefei Anhui230000)

机构地区：[1]安徽医科大学附属妇幼保健院,安徽合肥230001

出　　处：《哈尔滨医药》2023年第2期1-4,共4页Harbin Medical Journal

基　　金：安徽省自然科学基金项目(2108085MH260)。

摘　　要：目的分析子痫前期(preeclampsia,PE)相关差异表达miRNAs,选择miR-152预测其靶基因并进行生物信息学分析,探讨其参与子痫前期发病的分子机制。方法于GEO数据库中选择子痫前期胎盘组织miRNA差异表达数据集GSE103542,选择上调最显著的miR-152作为研究对象,应用miRDB、TargetScan及miRTarBase软件预测其靶基因并取交集;利用DAVID网络在线富集工具对交集靶基因进行GO功能注释及KEGG通路富集分析,并利用STRING网站对其进行蛋白质互作分析。结果获得的418个交集靶基因在生物过程(biological process,BP)上主要涉及生物黏附、细胞黏附及细胞发育调控等;在细胞组成(cellular component,CC)上主要涉及质膜组成部分、网格蛋白囊泡等;在分子功能(molecular function,MF)上主要涉及钙离子结合、离子结合等。KEGG信号通路富集分析显示其主要参与了调控干细胞多能性的信号通路及TGF-信号通路。蛋白互作分析显示IGF1R、SMAD3、RPS6KB1及PPP2CA是蛋白互作网络的关键节点。结论miR-152可能通过TGF-信号通路多方面影响滋养细胞增殖、侵袭及凋亡参与子痫前期的发生发展,本研究可为后续的靶基因验证及机制探索提供理论基础及研究思路。Objective To analyze the differentially expressed miRNAs associated with preeclampsia,select miR-152 predicted target genes and perform bioinformatics analysis to explore the molecular mechanisms involved in the pathogenesis of preeclampsia.Methods The most significantly upregulated miR-152 in the dataset GSE103542 was selected as the study target,and its target genes were predicted and taken for intersection.GO functional annotation and KEGG pathway enrichment analysis of the intersecting target genes were performed using the DAVID network online enrichment tool,and the STRING website was used to analyze their protein interactions.Results The 418 intersecting target genes obtained are mainly involved in biological processes such as bioadhesion,cell adhesion and cell developmental regulation;in cell composition,they are mainly involved in plasma membrane components,lattice protein vesicles;in molecular functions,they are mainly involved in calcium binding,ion binding.KEGG signaling pathway enrichment analysis showed that it was mainly involved in signaling pathways regulating stem cell pluripotency and TGF signaling pathway.Protein interaction analysis showed that IGF1R,SMAD3,RPS6KB1 and PPP2CA were the key nodes of the protein interaction network.Conclusion miR-152 may affect trophoblast proliferation,invasion and apoptosis through the TGF signaling pathway in multiple ways in the development of pre-eclampsia.

关 键 词：子痫前期 miR-152 靶基因 生物信息学分析 

分 类 号：R734.2[医药卫生—肿瘤]