甘蓝型油菜SGR基因家族的全基因组鉴定与功能分析  

Genome-wide identification and functional analysis of SGR gene family in Brassica napus L.

作　　者：唐玉凤 姚敏 何昕 官梅[1] 刘忠松[1] 官春云[1] 钱论文 TANG Yu-Feng;YAO Min;HE Xin;GUAN Mei;LIU Zhong-Song;GUAN Chun-Yun;QIAN Lun-Wen(College of Agronomy,Hunan Agricultural University/Collaborative Innovation Center of Grain and Oil Crops in South China,Changsha 410128,Hunan,China)

机构地区：[1]湖南农业大学农学院/南方粮油作物协同创新中心,湖南长沙410128

出　　处：《作物学报》2023年第7期1829-1842,共14页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31801399);湖南省教育厅科学研究重点项目(21A0135)资助。

摘　　要：叶绿素是植物进行光合作用的一类绿色色素,对植物生长有着直接的影响。本研究利用生物信息学方法在全基因组水平上对甘蓝型油菜(Brassica napus)、白菜(Brassica rapa)、甘蓝(Brassica oleracea)和拟南芥(Arabidopsis thaliana)中滞绿基因(STAY-GREEN,SGR)家族成员进行分析发现,28个SGR基因大多数包含4个外显子,编码碱性蛋白。染色体定位和共线性分析显示,甘蓝型油菜SGR基因家族成员中不存在串联复制,SGR基因家族成员之间具有线性关系,高度同源,且在进化过程中非常保守。此外,利用60K SNP对203份半冬性甘蓝型油菜自交系的叶绿素含量进行全基因组关联分析(Genome-wide association study,GWAS),检测到2个单体型区域Chr.A01:6,193,165~6,317,757 bp和Chr.C01:9,059,861~9,906,618 bp携带的Bna SGR1a-A01和Bna SGR1-C01与叶绿素含量显著相关。同时,结合50份半冬性甘蓝型油菜重测序数据,对这2个单体型进行进区域关联分析,检测到1个SNP定位在Bna SGR1a-A01的外显子2区域,并与叶绿素含量显著关联。共表达网络分析结果验证了Bna SGR1a-A01与Bna SGR2-A03直接相连,与Bna SGR1-C01、Bna SGR1-A08、Bna SGR2-C03、Bna SGR1-C07、Bna SGRL-C06、Bna SGRL-A10等基因间接相连形成了一个网络体系,共同调节叶绿素含量。T2拟南芥转Bna SGR1a-A01基因超表达植株的叶绿素a、叶绿素b和总的叶绿素含量相比野生型显著降低,表明Bna SGR1调控叶绿素降解。本研究为油菜SGR基因的功能研究和利用奠定了基础。Chlorophyll is a kind of green pigment for plant photosynthesis,which has a direct effect on plant growth.In this study,bioinformatics methods were used to identify the members of SGR gene family in Brassica napus,Brassica rapa,Brassica oleracea,and Arabidopsis thaliana.Most of the 28 SGR genes contained four exons,encoding basic proteins.Chromosome mapping and syntenic analysis showed that there was no tandem duplication in the SGR gene family members of Brassica napus.SGR gene family members had a linear relationship,highly homologous in evolution,and very conserved in the evolutionary process.Moreover,a genome-wide association study(GWAS)of chlorophyll content was performed using a Brassica 60K Illumina Infinium SNP array in 203 Brassica napus accessions.Two haplotype regions(Chr.A01:6,193,165–6,317,757 bp and Chr.C01:9,059,861–9,906,618 bp)carrying two SGR genes(BnaSGR1a-A01 and BnaSGR1-C01)were detected,which were significantly associated with chlorophyll content.Meanwhile,the regional association analysis of 50 resequenced rapeseed inbred lines revealed that a SNP located in exon 2 of BnaSGR1a-A01 significantly associated with chlorophyll content.Co-expression network analysis revealed that BnaSGR1a-A01 were directly linked with BnaSGR2-A03,and indirectly linked with BnaSGR1-C01,BnaSGR1-A08,BnaSGR2-C03,BnaSGR1-C07,BnaSGRL-C06,and BnaSGRL-A10,thus forming a molecular network involved in the potential regulation of chlorophyll content.Chlorophyll a,chlorophyll b,and the total chlorophyll content of T2 Arabidopsis transgenic plants overexpressing BnasSGR1a-A01 were significantly lower than wild type,indicating that BnaSGR1a-A01 regulated chlorophyll degradation.This study laid a foundation for the functional research and utilization of SGR gene in Brassica napus L.

关 键 词：甘蓝型油菜 SGR基因 全基因组关联分析 叶绿素含量 

分 类 号：S565.4[农业科学—作物学]