circ_0000064靶向miR-503对甲状腺癌细胞生物学行为的影响  

Effects of circ_0000064 on the biological behavior of thyroid cancer cells by targeting miR-503

作　　者：龚烨[1] 刘思吟 余喜梅[1] GONG Ye;LIU Siyin;YU Ximei(Xinjiang Uygur Autonomous Region People's Hospital,Urumqi,830001,Xinjiang,China)

机构地区：[1]新疆维吾尔自治区人民医院乳腺甲状腺科,新疆乌鲁木齐830001

出　　处：《肿瘤药学》2023年第2期173-180,共8页Anti-Tumor Pharmacy

摘　　要：目的探讨环状RNA 0000064(circ_0000064)靶向微小RNA 503(miR-503)对甲状腺癌细胞生物学行为的影响。方法实时定量PCR(RT-qPCR)分析21例甲状腺癌组织和对应癌旁组织中circ_0000064和miR-503的表达水平。将circ_0000064小干扰RNA(si-circ_0000064)、miR-503模拟物、si-circ_0000064+miR-503抑制物(anti-miR-503)分别转染甲状腺癌SW579细胞,采用平板克隆形成实验、细胞计数试剂盒(CCK-8)法检测SW579细胞增殖,Transwell实验检测SW579细胞迁移和侵袭,流式细胞术检测SW579细胞凋亡,蛋白质印迹法检测上皮型钙黏蛋白(E-cadherin)和神经型钙黏蛋白(N-cadherin)表达量。双荧光素酶报告实验和RT-qPCR分析circ_0000064和miR-503的靶向调控关系。结果甲状腺癌组织中circ_0000064表达量显著高于癌旁组织(P<0.05),miR-503表达量显著低于癌旁组织(P<0.05)。沉默circ_0000064后SW579细胞活力、克隆形成数、迁移和侵袭数、N-cadherin蛋白表达量均显著降低(P<0.05),E-cadherin蛋白表达量显著增加(P<0.05)。过表达miR-503后SW579细胞活力、克隆形成数、迁移和侵袭数、N-cadherin蛋白表达量均显著降低(P<0.05),E-cadherin蛋白表达量显著增加(P<0.05)。miR-503是circ_0000064的靶基因,沉默circ_0000064可显著上调miR-503表达(P<0.05)。抑制miR-503表达可显著减弱沉默circ_0000064对SW579细胞生物学行为的影响(P<0.05)。结论沉默circ_0000064可通过上调miR-503抑制甲状腺癌细胞增殖、迁移和侵袭,诱导细胞凋亡,从而抑制甲状腺癌进展。Objective To investigate the effects of circular RNA 0000064(circ_0000064)targeting miR-503 on the bio-logical behavior of thyroid cancer cells.Methods The expression levels of circ_0000064 and miR-503 in the thyroid can-cer tissues and the corresponding adjacent tissues of 21 cases were assessed using real-time quantitative PCR(RT-qPCR).Circ_0000064 small interfering RNA(si-circ_0000064),miR-503 mimic,si-circ_0000064+miR-503 inhibitor(anti-miR-503)were transfected into thyroid cancer SW579 cells,respectively.The plate cloning formation test and cell count-ing kit(CCK-8)method were applied to calculate the proliferation of SW579 cells,and Transwell test was performed to de-tect the migration and invasion of SW579 cells.Flow cytometry was selected to detect the cell apoptosis,and Western blot-ting was employed to detect the expressions of epithelial cadherin(E-cadherin)and neural cadherin(N-cadherin)proteins.The targeted regulation relationship between circ_0000064 and miR-503 was confirmed by dual luciferase reporter assay and RT-qPCR.Results The expression of circ_0000064 was significantly higher in thyroid cancer tissues than in adjacent tissues(P<0.05),while the expression of miR-503 was significantly lower in thyroid cancer tissues than in adjacent tissues(P<0.05).The cell viability,colony formation numbers,migration and invasion numbers,and N-cadherin protein expression of SW579 cells were significantly decreased(P<0.05),while E-cadherin protein expression was significantly increased(P<0.05)after silencing circ_0000064.The cell viability,colony formation numbers,migration and invasion numbers,N-cad-herin protein expression of SW579 cells were significantly decreased(P<0.05),while E-cadherin protein expression was significantly increased(P<0.05)after overexpressing miR-503.miR-503 was the target gene of circ_0000064,and silenc-ing circ_0000064 significantly increased the expression of miR-503(P<0.05).miR-503 inhibition significantly weakened the effects of silencing circ_0000064 on the biological behavior of

关 键 词：甲状腺癌 circ_0000064 miR-503 细胞增殖 凋亡 迁移 侵袭 

分 类 号：R736.1[医药卫生—肿瘤]