慢性疲劳综合征动物模型的构建与评价  

Establishment and Evaluation of Animal Models of Chronic Fatigue Syndrome

作　　者：李悦 韩秀珍[1] 徐秋露 商洪涛[1] LI Yue;HAN Xiuzhen;XU Qiulu;SHANG Hongtao(The Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院,南京210029

出　　处：《中国实验方剂学杂志》2023年第9期234-242,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省老年医学临床技术应用研究项目(LD2021004)。

摘　　要：慢性疲劳综合征(CFS)是一种多系统、多脏器功能失调的异质性疾病,其病因病机尚未完全阐明,治疗也缺乏特异性,建立符合CFS发病特点及内在机制的动物模型是对疾病开展研究的重要环节。现有CFS造模方法繁杂而不统一,笔者通过整理相关文献,从免疫模型、应激模型及病证结合中医模型3个方面对模型的构建方法、成模标准、机制及临床吻合度进行评价分析。其中免疫模型注重从病理生理角度进行构建,操作简便且研究广泛,能较好地模拟CFS病理特征,展开病机研究,但实验可重复性一般。应激造模是目前包括CFS在内多种神经精神疾病的常用造模方法,应激源种类广泛,可用于探究CFS的病因,但具有一定不可预测性。与前述2种西医模型相比,病证结合中医模型将现代医学与中医理论结合,临床吻合度高且实用价值大,但目前关于CFS的病证结合模型尚处于探索阶段,模型种类少,故仍有待进一步完善,以期建立科学合理、简便高效的动物模型,为探究CFS病因、发病机制和新的治疗思路等提供帮助。Chronic fatigue syndrome(CFS)is a heterogeneous disease with dysfunction in multiple systems and multiple organs.Its etiology and pathogenesis have not been fully clarified,and its treatment also lacks specificity.The key to studying CFS is developing animal models that reflect the underlying mechanisms and etiology of CFS.The existing CFS modeling methods are complicated and not unified.By sorting out relevant literature,the present study evaluated the modeling methods,modeling standards,mechanisms,and clinical coincidence of the immune model,the stress model,and the disease-syndrome combination model in traditional Chinese medicine(TCM).The immune model is mainly constructed from the perspective of pathophysiology,with easy operation and wide investigation,which can simulate the pathological characteristics of CFS to ensure pathogenesis research,but the experimental repeatability is general.Stress modeling is a common method for a variety of neuropsychiatric diseases,including CFS.Many different stressors can be employed to investigate the etiology of CFS,but their effects are unpredictable.Compared with the two western medicine models mentioned above,the TCM disease-syndrome combination model integrates modern medicine with TCM theory,with high clinical coincidence and great practical value.However,the TCM disease-syndrome combination model of CFS is still in the exploratory stage with a few types of models,which needs to be further improved,aiming to establish scientific,reasonable,simple,and efficient animal models to provide support for exploring the etiology,pathogenesis,and new treatment ideas of CFS.

关 键 词：慢性疲劳综合征 动物模型 免疫诱导 应激 病证结合 模型评价 

分 类 号：R22[医药卫生—中医基础理论] R-332[医药卫生—中医学] R775R965.1