长链基因间非编码RNA-p21通过调控YAP表达促进胃癌细胞凋亡  

Long intergenic non-coding RNA-p21 induces gastric cancer cells apoptosis through YAP expression regulation

作　　者：陈莹[1] 江雪[1] 倪彭智 郑玲燕 严丹方[1] 严森祥[1] CHEN Ying;JIANG Xue;NI Pengzhi;ZHENG Lingyan;YAN Danfang;YAN Senxiang(Department of Radiation Oncology,the First Affiliated Hospital,Zhejiang University School of Medicine,Zhejiang Hangzhou 310003,China;Department of Thoracic Surgery,the First Affiliated Hospital,Zhejiang University School of Medicine,Zhejiang Hangzhou 310003,China)

机构地区：[1]浙江大学医学院附属第一医院放疗科,浙江杭州310003 [2]浙江大学医学院附属第一医院胸外科,浙江杭州310003

出　　处：《现代肿瘤医学》2023年第10期1775-1780,共6页Journal of Modern Oncology

基　　金：浙江省自然科学基金资助项目(编号:LQ19H160033)。

摘　　要：目的:探讨lincRNA-p21在胃癌细胞凋亡中的作用及其潜在机制。方法:在胃癌MGC-803细胞系中分别转染siRNA以及过表达质粒实现下调/上调lincRNA-p21表达。lincRNA-p21和YAP的mRNA水平由qRT-PCR方法测定。之后通过流式细胞术和TUNEL测定对细胞凋亡进行检测,并用Western blot检测细胞凋亡相关蛋白。结果:上调lincRNA-p21促进胃癌细胞凋亡,反之亦然。研究还发现下调lincRNA-p21会引起YAP mRNA及蛋白表达量升高。最重要的是,由lincRNA-p21下调引起的抑制凋亡作用可以通过敲低YAP表达来解除。结论:lincRNA-p21可以通过调控YAP表达促进胃癌细胞凋亡,这可能为胃癌治疗提供新思路。Objective:To explore the role of lincRNA-p21 in gastric cancer(GC)cells apoptosis and the underlying mechanisms.Methods:The siRNA and overexpressing plasmid were transfected to MGC-803 cells down or up regulate lincRNA-p21 expression.The mRNA level of lincRNA-p21 and YAP was assessed by qRT-PCR.Cell apoptosis was quantitated by flow cytometry and TUNEL assay.Apoptosis associated proteins were detected by Western blot.Results:The lincRNA-p21 upregulation induced gastric cancer cells apoptosis,and vice versa.We also found that the expression of YAP mRNA and protein was increased by downregulated lincRNA-p21.Moreover,the anti-apoptotic effect caused by lincRNA-p21 downregulation can be attenuated by the knocking down of YAP expression.Conclusion:lincRNA-p21 could serve as the cells apoptosis inducer in GC by regulation YAP expression,which sheds new light on gastric cancer treatment.

关 键 词：胃癌 长链基因间非编码RNA-p21 凋亡 YAP蛋白 

分 类 号：R735.2[医药卫生—肿瘤]