昆明小鼠Lewis肺癌细胞种植后对心率变异的影响  

Effects of Lewis lung cancer cell implantation on heart rate variability in Kunming mice

作　　者：刘雪莹 李炜健 凌永健 辜晓康 刘桢昀 张浩烽 何雷[2] 陈伟强 LIU Xueying;LI Weijian;LING Yongjian;GU Xiaokang;LIU Zhenyun;ZHANG Haofeng;HE Lei;CHEN Weiqiang(Southern Medical University,Guangdong Guangzhou 510280,China;Department of Endocrinology,Zhujiang Hospital of Southern Medical University,Guangdong Guangzhou 510280,China;Department of EKG Room,Zhujiang Hospital of Southern Medical University,Guangdong Guangzhou 510280,China)

机构地区：[1]南方医科大学第二临床医学院,广东广州510280 [2]南方医科大学珠江医院内分泌科,广东广州510280 [3]南方医科大学珠江医院心电图室,广东广州510280

出　　处：《现代肿瘤医学》2023年第10期1816-1821,共6页Journal of Modern Oncology

基　　金：大学生创新训练项目(编号:202112121187)。

摘　　要：目的:研究Lewis肺癌小鼠心率变异性(HRV)指标的变化规律,探索与肺癌诊断相关的心率变异指标。方法:将70只雄性昆明小鼠随机分为对照组(正常小鼠)20只和实验组(Lewis肺癌建模组)50只,实验组在建模之后根据是否成瘤分为未成瘤组和成瘤组。在建模后分别记录各组小鼠的HRV指标数值和统计分析。结果:建模后成瘤组有36只小鼠,未成瘤组14只,包括对照组在内各组小鼠均存活。由单因素ANOVA检验和Kruskal-Wallis检验得到差异具有统计学意义(P<0.05)的指标有sdnn、rmssd、lf、hf,且在三组的两两比较中,sdnn的差异均具有统计学意义(P<0.05)。进行Spearman相关分析得:sdnn、rmssd、lf、hf与肿瘤都具有一定的负相关关系(P<0.05)。多项Logistic回归分析得sdnn和rmssd在对照组和成瘤组、未成瘤组和成瘤组中产生显著性影响(P<0.05)。在决策树模型中,sdnn的特征重要性在差异具有统计学意义的4个指标中都是最高的,rmssd次之。在三组中进行ROC曲线分析,sdnn和rmssd对于成瘤均有很高的诊断价值,得到sdnn截止水平为37.49 ms,敏感性为97.2%,特异性为91.2%;rmssd的截止水平为52.82 ms,敏感性为94.4%,特异性为79.4%。结论:低水平的sdnn和rmssd可能是Lewis肺癌移植瘤的独立危险因素,HRV中sdnn、rmssd、hf等下降能够反映自主神经紊乱,后者会影响肿瘤的进展。Objective:To investigate the variation rule of heart rate variability(HRV)indexes in Lewis lung cancer mice,explore the HRV indexes for lung cancer diagnosis.Methods:70 male Kunming mice were randomly divided into a normal group with 20 mice and an experimental group(Lewis lung cancer model)with 50 mice.The experimental group was divided into the non-tumor-forming group and the tumor-forming group according to whether the modeling was successful.The HRV index values and statistical analysis of the mice in each group were recorded after modeling.Results:After modeling,there were 36 mice in the tumor formation group and 14 mice in the non-tumor formation group.All mice survived,including the control group.sdnn,rmssd,lf,and hf were statistically significant(P<0.05)by one-way ANOVA test and Kruskal-Wallis test,and the differences in sdnn among the three groups were statistically significant(P<0.05).Spearman correlation analysis showed that sdnn,rmssd,lf,hf had a certain negative correlation with tumor(P<0.05).Multivariate Logistic regression analysis showed that sdnn and rmssd had a significant effect on the control group and the tumor group,and the non-tumor group and the tumor group(P<0.05).In the decision tree model,the feature importance of sdnn was the highest among the four indicators with statistically significant differences,followed by rmssd.ROC curve analysis showed that sdnn and rmssd had high diagnostic value for tumorigenesis in the three groups.The cut-off level of sdnn was 37.49 ms,the sensitivity was 97.2%,and the specificity was 91.2%.The cut-off level of rmssd was 52.82 ms,the sensitivity was 94.4%,and the specificity was 79.4%.Conclusion:Low levels of sdnn and rmssd may be independent risk factors for Lewis lung cancer transplantation.The decline of sdnn,rmssd,and hf in HRV can reflect autonomic disorder,which affects tumor progression.

关 键 词：SDNN rmssd LEWIS肺癌 心率变异性 自主神经系统 昆明小鼠 

分 类 号：R734.2[医药卫生—肿瘤]