两种不同造模方法形成的酒精性肝病小鼠模型比较  

Comparison of alcoholic liver disease mice model established by two different ways

作　　者：谢超 王晓凡 刘天会[1] 王萍[1] 范旭[1] 刘琳[1] 丛敏[1] XIE Chao;WANG Xiao-fan;LIU Tian-hui(Liver Research Center,Beijing Friendship Hospital,Capital Medical University,Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis,National Clinical Research Center of Digestive Disease,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院肝病中心,肝硬化转化医学北京市重点实验室暨国家消化系统疾病临床医学研究中心,北京100050

出　　处：《临床和实验医学杂志》2023年第6期561-565,共5页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(编号:82170614);王宝恩肝纤维化基金(编号:CFHPC 2021042)。

摘　　要：目的明确两种不同造模方法对酒精性肝病模型小鼠的肝脏脂变、炎症及铁沉积的影响。方法分别采用酒精液体饮食喂养C57BL/6小鼠10 d和8周,分为10 d对照组(对照饮食)、10 d模型组(酒精液体饮食)、8周对照组(对照饮食)、8周模型组(酒精液体饮食),每组10只。造模结束后留取各组小鼠血清及肝脏组织检测。采用苏木精-伊红、油红O、普鲁士蓝染色分别观察小鼠肝脏炎症、脂变、铁沉积情况。依据相应试剂盒说明书检测血清中肝功能生化指标[天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)]、脂质相关生化指标[血清甘油三脂和总胆固醇]、铁代谢相关生化指标[血清铁调素、血清铁及总铁结合力(TIBC)、转铁蛋白饱和度(TS)]。采用Real-time PCR(qPCR)检测肝脏炎症相关基因IL-1β、IL-10以及铁代谢相关基因HAMP的表达。结果苏木精-伊红染色显示10 d和8周模型组仅少量炎症细胞浸润;油红O染色显示10 d及8周模型组较对照组均出现明显脂变;普鲁士蓝染色显示10 d及8周模型组较对照组均无明显铁沉积。10 d模型组的血清ALT、AST水平以及IL-1β表达均较10 d对照组升高,差异均有统计学意义(P<0.05),两组间IL-10表达差异无统计学意义(P>0.05)。8周模型组血清ALT、AST水平以及IL-1β、IL-10表达与8周对照组比较,差异均无统计学意义(P>0.05)。10 d模型组甘油三酯、总胆固醇水平均较10 d对照组升高,差异均有统计学意义(P<0.05);8周模型组甘油三酯、总胆固醇与对照组比较,差异均无统计学意义(P>0.05)。两组模型中血清铁均较对照组均有一定上升趋势,但差异均无统计学意义(P>0.05)。10 d模型组与10 d对照组TS水平比较,差异无统计学意义(P>0.05);8周模型组TS水平较8周对照组升高,差异有统计学意义(P<0.05)。10 d及8周模型组的TIBC水平与对照组比较,差异均无统计学意义(P>0.05)。10 d模型组与10 d对照组血清�Objective To investigate the effects of two different modeling methods on liver inflammation,steatosis,and iron deposition in alcoholic liver disease mice.Methods C57BL/6 mice were fed with alcohol liquid diet for 10 days and 8 weeks respectively,and were divided into 10 day control group(control diet),10 day model group(alcoholic liquid diet),8 week control group(control diet),8 week model group(alcoholic liquid diet),with 10 mice in each group.Serum and liver tissues were harvested for further study.The level of mice hepatic inflammation,steatosis,and iron deposition were evaluated by hematoxylin-eosin staining,oil red O staining,and prussian blue staining.The corresponding test kits were used to detect the level of the liver functional markers[aspartate aminotransferase(AST)and alanine aminotransferase(ALT)]and the lipid metabolism associated biomarkers[triacylglycerol,total cholesterol]and the iron metabolism associated biomarkers[hepcidin,iron and total iron binding capacity(TIBC),transferrin saturation(TS)]in serum.Real-time PCR(qPCR)was used to detect the expression of inflammation and iron metabolism-related genes:IL-1β,IL-10 and HAMP.Results According to the hematoxylin-eosin staining,no apparent inflammatory cell infiltration was found in livers from 10 days and 8 weeks ethanol model compared to those from the control group.Oil red O staining showed that hepatic steatosis occurred in both 10 days and 8 weeks model group compared to those of the control groups.Meanwhile,no conspicuous liver iron deposition could be observed in livers from10 days and 8 weeks model group mice.The levels of serum ALT,AST and IL-1βin the 10 days model group were significantly higher than those in the 10 days control group,the differences were statistically significant(P<0.05),there was no significant difference in the expression of IL-10 between the two groups(P>0.05).There was no significant difference in serum ALT,AST,IL-1β,IL-10 expression between the 8 weeks model group and the 8 weeks control group(P>0.05).The levels

关 键 词：酒精 小鼠模型 脂肪肝 

分 类 号：R575[医药卫生—消化系统] R-332[医药卫生—内科学]