1例云南汉族大肠癌中NDRG1全长基因扩增及特征分析  

Amplification and characterization of full-length NDRG1 gene in a case of colorectal cancer of Yunnan Han nationality

作　　者：杜娟 沈红 王芳 廖文平 王开昕 DU Juan;SHEN Hong;WANG Fang;LIAO Wenping;WANG Kaixin(Department of Pathology,the First People´s Hospital of Hefei,Hefei 230001,China;Department of Pathology and Pathophysiology,School of Basic Medicine,Kunming Medical University,Kunming 650500,China;Department of Pathology,the First People´s Hospital of Zhaotong,Kunming 657000,China;Laboratory of toxicity Pathology,Drag safety evaluation center of Yunnan institute of Materia Medica,Kunming 650111,China;Department of Pathology,Huazhong University of Science and Technology Union Shenzhen Hospital,Shenzhen 518052,China)

机构地区：[1]合肥市第一人民医院病理科,安徽合肥230601 [2]昆明医科大学基础医学院病理学与病理生理学系,云南昆明650500 [3]昭通市第一人民医院病理科,云南昭通657000 [4]云南省药物研究所安全性评价中心毒性病理研究室,云南昆明650111 [5]华中科技大学协和深圳医院病理科,广东深圳518052

出　　处：《山东第一医科大学（山东省医学科学院）学报》2023年第4期268-272,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：国家自然科学基金(81260361)。

摘　　要：目的分析云南汉族大肠癌与高加索人大肠癌细胞中N-myc下游调节基因(N-myc down-stream regulated gene 1,NDRG1)的差异。方法收集云南汉族大肠癌患者的腹水,进行癌细胞培养及传代,同时选择高加索人大肠癌细胞系Caco2进行培养。通过实时荧光定量PCR,在mRNA水平观察NDRG1在云南汉族及高加索人大肠癌细胞中的表达特点。结果云南汉族与高加索人大肠癌细胞NDRG1全长序列一致,云南汉族大肠癌中NDRG1 mRNA的表达明显高于高加索人。结论NDRG1基因在云南汉族及高加索人大肠癌细胞中核酸序列相同,但mRNA表达水平存在一定差异。Objective:To explore the difference of NDRG1 gene between the Han nationality in Yunnan and Caucasian colorectal cancer cells.Methods:Ascites from Yunnan Han nationality patients with colorectal cancer were collected for cancer cell culture and passage,and Caucasian colorectal cancer cell line Caco2 was selected for culture.The expression characteristics of NDRG1 in Yunnan Han nationality and Caucasian colorectal cancer cells were observed at mRNA level by real-time fluorescence quantitative PCR.Results:The full-length sequence of NDRG1 in a case of Yunnan Han nationality and Caucasian colorectal cancer cells was consistent,the expression of NDRG1 mRNA in colorectal cancer of Yunnan Han nationality was significantly higher than that of Caucasian.Conclusion:It is speculated that the nucleic acid sequence of NDRG1 gene are the same in Yunnan Han nationality and Caucasian colorectal cancer cells,but the mRNA expression level is different.

关 键 词：NDRG1 云南 汉族 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]