敲低lncRNA CACNA1C-AS2促进上皮间质转化增强人食管癌细胞的增殖、侵袭和迁移  

Knockdown of IncRNA CACNA1C-AS2 promotes epithelial-mesenchymal transition and enhancess the proliferation,invasion and migration of human esophageal cancer cells

作　　者：秦玉萍 曹通 许颖 蒋旭 马佳[1] 禹莉[1,2] QIN Yuping;CAO Tong;XU Ying;JIANG Xu;MA Jia;YU Li(Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis,Bengbu Medical College,Bengbu 233000,China;Department of Transfusion,School of Laboratory Medicine,Bengbu Medical College,Bengbu 233000,China)

机构地区：[1]蚌埠医学院肿瘤基础研究与临床检验诊断重点实验室,安徽蚌埠233000 [2]蚌埠医学院检验医学院输血学教研室,安徽蚌埠233000

出　　处：《细胞与分子免疫学杂志》2023年第3期249-257,共9页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家大学生创新创业培训计划(201810367029);安徽省自然科学基金(1808085MH305);省级大学生创新创业培训计划(S202010367032)。

摘　　要：目的 探讨钙电压门控通道亚基α1C反义RNA2(CACNA1C-AS2)通过调节上皮间质转化(EMT)对食管癌细胞恶性生物学行为的影响。方法 通过癌症基因组图谱(TCGA)数据库分析CACNA1C-AS2在癌旁组织和食管癌组织的表达;实时定量PCR检测食管癌细胞中CACNA1C-AS2 mRNA表达水平;在食管癌细胞中敲低和高表达CACNA1C-AS2后,采用噻唑蓝(MTT)法和细胞集落形成实验检测细胞的活力,TranswellTM小室法检测细胞的侵袭能力和纵向迁移能力,划痕愈合实验检测细胞的横向迁移能力,流式细胞术检测细胞的凋亡率;Western blot法检测神经钙黏素(N-cadherin)、波形蛋白(vimentin)和锌指转录因子snail 2(slug)蛋白表达。结果 CACNA1C-AS2在食管癌组织和细胞系中均低表达;敲低EC-9706细胞和Eca-109细胞中CACNA1C-AS2后,食管癌细胞的侵袭、迁移能力和细胞活力显著增强,细胞凋亡减少;N-cadherin、vimentin和slug表达上调。过表达CACNA1C-AS2则结果相反。结论 CACNA1C-AS2通过促进EMT增强食管癌细胞的增殖、侵袭和迁移。Objective To investigate the effect of calcium voltage gated channel subunitα1C antisense RNA2(CACNA1C-AS2)on malignant biological characteristics of esophageal cancer cells by regulating epithelial mesenchymal transition(EMT).Methods CACNA1C-AS2 expression levels in paracancerous tissues and esophageal cancer tissues were analyzed by TCGA database.Real-time quantitative PCR was used to detect the expression of CACNA1C-AS2 mRNA in esophageal cancer cells.Following the knockdown and high expression of CACNA1C-AS2 in esophageal cancer cells,the viability of the cells was tested by MTT assay and cell colony formation assay.TranswellTM chamber method was used to measure the invasion and longitudinal migration of the cells.The horizontal migration abilty of the cells was evaluated by wound healing test.The apoptosis rates of cells were detected by flow cytometry.Western blot analysis was used to detect the expressions of N-cadherin,vimentin and slug.Results CACNA1C-AS2 expression levels were low in esophageal cancer tissues and cellines.After knocking down the expression of CACNA1C-AS2 in EC-9706 cells and Eca-109 cells,the ability of invasion and migration and viability of esophageal cancer cells were significantly enhanced,and the apoptosis rates were decreased,while the expressions of N-cadherin,vimentin and slug were up-regulated.However,the results are opposite via the over-expression of CACNA1C-AS2.Conclusion CACNA1C-AS2 enhances the proliferation,invasion and migration of esophageal cancer cells by promoting EMT.

关 键 词：食管癌 长链非编码RNA(lncRNA) 钙电压门控通道亚基α1C反义RNA2(CACNA1C-AS2) 上皮间质转化(EMT) 

分 类 号：R735.1[医药卫生—肿瘤] R73-3[医药卫生—临床医学]