NLRP3炎症小体在颞下颌关节骨关节炎痛觉过敏中的作用  

Involvement of NLRP3 inflammasome in hyperalgesia induced by temporomandibular joint osteoarthritis

作　　者：白苗苗 沈振国 丁春萌 邢田[1] 侯爱兵[1] Bai Miaomiao;Shen Zhenguo;Ding Chunmeng;Xing Tian;Hou Aibing(Stomatological College of Anhui Medical University,The Affiliated Stomatological Hospital of Anhui Medical University,Key Lab of Oral Diseases Research of Anhui Province,Hefei 230032)

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究中心实验室,合肥230032

出　　处：《安徽医科大学学报》2023年第4期655-660,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省重点研究与开发计划项目(编号:1704f0804025)。

摘　　要：目的 探究核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)炎症小体在大鼠颞下颌关节骨关节炎(TMJOA)痛觉过敏中的作用。方法 选取20只6周龄雄性SD大鼠随机分为NS组和MIA组,采用碘乙酸钠(MIA)注射颞下颌关节上腔构建实验性TMJOA大鼠模型;采用Von Frey检测大鼠颞下颌关节(TMJ)区注射MIA后痛觉阈值变化;苏木精-伊红(HE)、番红固绿染色观察髁突结构的改变;HE染色观察三叉神经节(TG)的病理结构改变;免疫组化检测TG NLRP3蛋白表达及分布;Western blot检测TG NLRP3、白细胞介素(IL)-1β蛋白表达;实时荧光定量反转录聚合酶链式反应(qRT-PCR)检测TG NLRP3、凋亡相关斑点状蛋白(ASC)、半胱氨酸蛋白酶1(Caspase-1)、IL-1β、IL-18 mRNA表达。结果 与NS组比较,实验性TMJOA大鼠痛觉阈值下降(P<0.05);TMJ、TG病理结构改变明显;TMJOA组大鼠TG组织中表达NLRP3的蛋白和mRNA水平均升高(P<0.05)。结论 NLRP3炎症小体可能参与调控TMJOA大鼠痛觉过敏。Objective To investigate the role of nucleotidebinding oligomerization domain-like receptor protein 3(NLRP3)inflammasome in hyperalgesia induced by temporomandibular joint osteoarthritis(TMJOA)in rats.Methods Twenty 6-week-old male SD rats were randomly divided into NS group and MIA group.The rat model of TMJOA was established by injection monosodium iodoacetate(MIA)into the upper cavity of temporomandibular joint(TMJ).The changes of pain threshold in the TMJ region of rats after MIA injection were detected by Von Frey.The changes of condyle structure were observed by Hematoxylin-eosin(HE)and Safranin O-fast green stains.Histopathological changes of trigeminal ganglion(TG)were observed by HE stains.The expression and distribution of TG NLRP3 protein were detected by immunohistochemistry.Western blot was used to detect the protein levels of NLRP3 and interleukin(IL)-1βin TG.The mRNA levels of NLRP3,apoptosis-associated speck-like protein(ASC),cysteinyl aspartate specific proteinase(Caspase-1),IL-1βand IL-18 in TG were detected by quantitative real-time polymerase chain reaction(qRT-PCR).Results Compared to saline group,the pain threshold of experimental TMJ osteoarthritis rats decreased(P<0.05).TMJ and TG showed obvious pathological changes.The protein and mRNA levels of NLRP3 expressed in the tissues of rats in the TMJOA group increased(P<0.05).Conclusion NLRP3 inflammasome may be involved in the regulation of hyperalgesia in TMJOA rats.

关 键 词：NLRP3炎症小体 颞下颌关节骨关节炎 痛觉过敏 三叉神经节 

分 类 号：R782.6[医药卫生—口腔医学]