4D label-free proteomic analysis of vitreous from patients with rhegmatogenous retinal detachment  

作　　者：Qiu-Yi Huo Meng-Chao Zhu Wen-Chao Yang Yi-Peng Wang Song Chen 

机构地区：[1]Wenzhou Medical University,Wenzhou 325027,Zhejiang Province,China [2]Anyang Eye Hospital,Anyang 455000,Henan Province,China [3]Tianjin Eye Hospital,Tianjin Eye Institute,Tianjin Key Lab of Ophthalmology and Visual Science,Nankai University Affiliated Eye Hospital,Tianjin 300020,China

出　　处：《International Journal of Ophthalmology(English edition)》2023年第4期523-531,共9页国际眼科杂志（英文版）

摘　　要：AIM:To identify metabolites,proteins,and related pathways involved in the etiology of rhegmatogenous retinal detachment(RRD)for use as biomarkers in diagnosing and treating RRD.METHODS:Vitreous specimens were collected and liquid chromatography-tandem mass spectrometry analysis was per formed using the four-dimensional label-free technique.Statistically significant differentially expressed proteins,gene ontology(GO)terms,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway representations,and protein interactions were analyzed.RESULTS:Nine specimens were subjected to proteomic analysis.In total,161 proteins were identified as differentially expressed proteins(DEPs),including 53 upregulated proteins and 108 downregulated proteins.GO functional analysis revealed that some DEPs were enriched in neuron-related terms and membrane protein terms.Moreover,KEGG analysis indicated that the cell adhesion molecule metabolic pathway was associated with the greatest number of DEPs.Finally,the evaluation of protein-protein interaction network revealed that DEPs were clustered in neuronal adhesion,apoptosis,inflammation and immune responses,correct protein folding,and glycolysis.CONCLUSION:Proteomic profiling is useful for the exploration of molecular mechanisms that underlie RRD.This study reveals increased expression levels of proteins related to heat shock protein content,glycolysis,and inflammatory responses in RRD.Knowledge regarding biomarkers of RRD pathogenesis may help to prevent the occurrence of RRD in the future.

关 键 词：VITREOUS rhegmatogenous retinal detachment PROTEOME 4D label-free 

分 类 号：R774.12[医药卫生—眼科]