Simultaneous Determination of Six Components in Cortex Phellodendri by HPLC  

作　　者：Lian LIAN Guosheng WAN Weili JIA Huiyuan GAO 

机构地区：[1]School of Biological and Chemical Engineering,Liaoning Institute of Science and Technology,Benxi 117004,China [2]College of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Agricultural Biotechnology》2023年第2期87-90,共4页农业生物技术（英文版）

摘　　要：[Objectives]This study was conducted to establish the method for simultaneous determination of six active components.[Methods]Simultaneous determination of chlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine,palmatine and berberine in Cortex Phellodendri was carried out by HPLC with a Diamonsil C18(4.6 mm×250 mm,5μm)column was used.The mobile phase was acetonitrile-water(1‰acetic acid,2 mmol ammonium acetate)solution in gradient elution.The detection wavelength was set at 280 nm,and the column temperature was kept at 25℃and the flow rate was 1 ml/min.[Results]The linear ranges of chlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine,palmatine and berberine were 20.00-320.00,18.75-130.00,25.00-200.00,5.00-100.00,20.00-200.00,and 0.09-1.80 mg/L,respectively.The average recovery was 98.1%,99.4%,97.5%,97.3%,104.0%,and 98.5%,respectively;and the RSDs were 0.5%,0.6%,0.8%,1.0%,1.4%,and 0.9%,respectively.[Conclusions]The method is convenient,stable,reliable and suitable for quality control of Cortex Phellodendri.

关 键 词：Cortex Phellodendri Chlorogenic acid Phellodendrine MAGNOFLORINE JATRORRHIZINE PALMATINE BERBERINE Determination 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]