不同叶龄红小豆叶片的转录组分析  

Transcriptome analysis of adzuki bean leaves at different ages

作　　者：董伟欣[1,2] 张磊 李东晓 张月辰[1] DONG Weixin;ZHANG Lei;LI Dongxiao;ZHANG Yuechen(College of Agronomy/State Key Laboratory of Crop Improvement and Regulation in North China,Hebei Agricultural University,Baoding,Hebei 071000,China;Hebei Open University,Shijiazhuang,Hebei 050080,China)

机构地区：[1]河北农业大学农学院/华北作物改良与调控国家重点实验室,河北保定071000 [2]河北开放大学,河北石家庄050080

出　　处：《福建农林大学学报（自然科学版）》2023年第3期285-293,共9页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：河北省自然科学基金项目(C2021204045);国家现代农业产业技术体系项目(CARS-08-G-22)。

摘　　要：为揭示红小豆苗期不同叶龄基因表达量的变化,以晚熟品种‘唐山红小豆’为材料,通过RNA-Seq技术对3叶龄和6叶龄红小豆叶片进行转录组比较分析.结果表明:两个样本的转录组测序共得到94.1×10^(6)个原始reads,过滤后得到92.31×10^(6)个clean reads,Q30比例为94.17%,GC含量为43.68%,能定位到基因组序列上的碱基数占97.77%,在参考序列上有唯一比对序列数占93.07%;两个样本共获得6314个差异表达基因,其中,3059个基因上调表达,355个基因下调表达,对差异基因进行GO和KEGG分析,发现差异表达基因主要富集在光信号接收、昼夜节律、光合作用—天线蛋白、淀粉蔗糖和植物激素信号转导等代谢途径上,其中植物激素信号转导代谢途径中的差异表达基因数目最多,达108个;从昼夜节律、天线蛋白和植物激素信号转导代谢途径中各选取2个差异显著的基因进行验证,结果与FPKM值趋势一致.红小豆转录组测序数据准确可靠,苗期生长发育受到光合作用、淀粉和蔗糖、昼夜节律和植物激素等代谢途径的调控,为红小豆的开花结荚提供保障.该结果为进一步研究红小豆苗期生长过程中相关基因的动态变化提供依据.To reveal the changes in gene expressions of Vigna angularis(adzuki bean)during seedling stage,comparative transcriptomic analysis of leaves from late maturing variety of‘Tangshan adzuki bean'grown at 3-and 6-leaf stages was performed by RNA-Seq.A total of 92.31×10^(6)clean reads were filtered from 94.1×10^(6)raw reads with a Q30 value of 94.17%and GC content of 43.68%.The results covered 97.77%genomic sequences and 93.07%sequences were uniquely aligned to the reference genome.A total of 6314 differentially expressed genes(DEGs)were obtained,among which 3059 were up-regulated and 3255 were down-regulated.Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed that these DEGs mainly participated in the metabolic pathways concerning light signal reception,circadian rhythm,photosynthesis-antenna protein,metabolisms of starch and sucrose and plant hormone signaling,and the most number of DEGs(108)were detected from the pathways regulating plant hormone signaling.Two significant DEGs,selected from pathways related to circadian rhythm,antenna protein and phytohormone signaling were further validated by qRT-PCR,and the results were consistent with the trend of FPKM values.To summarize,the growth of adzuki bean seedling was regulated by metabolic pathways involving photosynthesis,starch and sucrose synthesis,circadian rhythm and phytohormones,which provide material bases for the flowering and pod development of adzuki bean.

关 键 词：红小豆 3叶龄和6叶龄叶片 转录组 实时荧光定量PCR 

分 类 号：S521.01[农业科学—作物学]