鹌鹑SLC24A5基因多态性与羽色性状的关联性分析  

Association Analysis Between SLC24A5 Gene Polymorphism and Feather Color Traits in Quail

作　　者：赵毅威 张小辉[1,2] 庞有志 苑志文[1] 户运奇 霍琳珂 任世威 ZHAO Yiwei;ZHANG Xiaohui;PANG Youzhi;YUAN Zhiwen;HU Yunqi;HUO Linke;REN Shiwei(College of Animal Science,Henan University of Science and Technology,Luoyang,Henan 471003;Luoyang Key Laboratory of Animal Genetic and Breeding,Luoyang,Henan 471003)

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]洛阳市动物遗传育种重点实验室,河南洛阳471003

出　　处：《中国家禽》2023年第5期7-12,共6页China Poultry

基　　金：河南省自然科学基金项目(202300410153);河南省科技攻关项目(202102110088)。

摘　　要：为研究SLC24A5基因与鹌鹑羽色性状的关系,试验采用RT-qPCR方法研究朝鲜鹌鹑和北京白羽鹌鹑在胚胎不同发育时期的mRNA表达水平。结果显示:SLC24A5基因在朝鲜鹌鹑胚胎发育时期的表达水平极显著高于北京白羽鹌鹑(P<0.01);鲜鹌鹑和北京白羽鹌鹑的3种基因型频率分布差异极显著(P<0.01)。位于外显子1的SNP1(c.A5T)和外显子6的SNP2(c.G703A)均为中性位点,但SNP2(c.G703A)位点造成的编码蛋白G235S位点突变并不保守,属于多变性位点。研究表明,SLC24A5基因与鹌鹑羽色性状有关联性,可以为鹌鹑羽色育种提供理论依据。In order to study the relationship between SLC24A5 gene and feather color traits of quail,the mRNA expression levels of Korean quail and Beijing white quail at different embryonic development stages were studied by RT-qPCR.The results showed that the expression level of SLC24A5 gene in Korean quail was significantly higher than that in Beijing white feather quail(P<0.01).According to KASP genotyping,there was a significant difference in the frequency distribution of three genotypes be⁃tween Korean quail and Beijing white quail(P<0.01),SNP1(c.A5T)located in exon 1 and SNP2(c.G703A)located in exon 6 were both neutral sites,but the mutation of G235S coding protein caused by SNP2(c.G703A)was not conservative and be⁃longed to polytropic sites.The results indicated that SLC24A5 gene was related to quail feather color traits,which could provide a theoretical basis for quail feather color breeding.

关 键 词：鹌鹑 羽色 SLC24A5基因 基因突变 

分 类 号：S839.2[农业科学—畜牧学]