一株类节杆菌协同还原六价铬和降解阿特拉津的研究  

Simultaneous Cr(Ⅵ)reduction and atrazine degradation by an isolated Paenarthrobacter sp.AT

作　　者：李祥龙 吴尚华[1,2] 樊浩楠 董钰珠 白志辉 庄绪亮[1,2,4] LI Xianglong;WU Shanghua;FAN Haonan;DONG Yuzhu;BAI Zhihui;ZHUANG Xuliang(Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085;University of Chinese Academy of Sciences,Beijing 100049;Xiong′an Institute of Innovation,Xiong′an New Area 071899;The Institute of Tibetan Plateau Research,Chinese Academy of Sciences,Beijing 100101)

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]中国科学院大学,北京100049 [3]雄安创新研究院,雄安新区071899 [4]中国科学院青藏高原研究所,北京100101

出　　处：《环境科学学报》2023年第4期236-242,共7页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.41907273,91951108);国家重点研发计划(No.2019YFC1805803);中国科学院战略性先导科技专项(No.XDA23010400);中国科学院国际伙伴计划(No.121311KYSB20200017)。

摘　　要：六价铬(Cr(Ⅵ))和阿特拉津复合污染在环境中普遍存在,给生态安全和人类健康带来严重危害.为了实现对Cr(Ⅵ)和阿特拉津复合污染的协同修复,本研究在复合污染场地筛选出一株同时具有阿特拉津降解能力和Cr(Ⅵ)还原能力的类节杆菌Paenarthrobacter sp.AT.在单一污染条件下,菌株AT可以在24 h内将初始浓度为400 mg·L^(-1)的阿特拉津完全降解,将初始浓度为10 mg·L^(-1)的Cr(VI)完全还原;同时,菌株还具有很强的阿特拉津和Cr(Ⅵ)耐受性.菌株AT被鉴定含有trz N、atz B和atz C阿特拉津降解功能基因,将阿特拉津降解为无毒终产物氰尿酸,同时生成乙胺和异丙胺两种代谢副产物,其中,乙胺在溶液中持续积累,而异丙胺则短暂积累再被降解.在复合污染条件下,菌株AT对初始浓度为200 mg·L^(-1)阿特拉津的降解率为100%,对初始浓度为10 mg·L^(-1)的Cr(Ⅵ)的还原率为44%.本研究证实了微生物可以利用有机污染物作为氮源实现对重金属的转化,为开发绿色低碳微生物修复技术提供了借鉴.Cr(Ⅵ)and atrazine always co-exist in polluted environment and cause huge threat to ecology safety and human health.In order to simultaneously remove Cr(Ⅵ)and atrazine,our study isolated an Paenarthrobacter sp.AT from the co-contaminated soil.For single pollutant,strain AT totally degraded 400 mg·L^(-1)atrazine or reduced 10 mg·L^(-1)Cr(Ⅵ)in 24 h.Strain AT was proved to be resistant to atrazine and Cr(Ⅵ).Strain AT was proved to have trzN,atzB,and atzC genes and could degrade atrazine to cyanuric acid,together with ethylamine and isopropylamine.Ethylamine was accumulated during atrazine degradation,while isopropylamine was temporarily accumulated and then was reused.For combined pollution,strain AT simultaneously degraded 100%of 200 mg·L^(-1) atrazine and reduced 44%of 10 mg·L^(-1)Cr(Ⅵ).Our study proved that bacteria could utilize the organic pollutant as nitrogen resource to transform heavy metal,which might help to develop the green and low-carbon bioremediation technology.

关 键 词：六价铬 阿特拉津 类节杆菌 微生物修复 复合污染 

分 类 号：X172[环境科学与工程—环境科学]