胶质母细胞瘤外泌体的作用机制研究进展  

The action mechanism of glioblastoma cell-derived exosome:a review

作　　者：黎娜 罗丽 杨雅婷[1] 刘兆梅 邱小燕[2] 王明玉 王炜[1] 肖雄[1] LI Na;LUO Li;YANG Yating;LIU Zhaomei;QIU Xiaoyan;WANG Mingyu;WANG Wei;XIAO Xiong(College of Veterinary Medicine,Southwest University,Chongqing 400715,China;College of Animal Science and Technology,Southwest University,Chongqing 400715,China;Institute of Laboratory Animal Science,Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)

机构地区：[1]西南大学动物医学院,重庆400715 [2]西南大学动物科学技术学院,重庆400715 [3]重庆市中药研究院实验动物研究所,重庆400065

出　　处：《生物工程学报》2023年第4期1477-1501,共25页Chinese Journal of Biotechnology

基　　金：中央高校基本科研业务费专项资金(XDJK2020B011);重庆市教委科学技术研究计划(KJZD-K202200208);国家自然科学基金(31572488,32072778);国家生猪技术创新中心项目(NCTIP-XD/C17);重庆市自然科学基金(cstc2021jcyj-msxmX0972);重庆市中药研究院基本科研业务费项目(cstc2019jxjl-jbky120002)。

摘　　要：胶质母细胞瘤(glioblastoma,GBM)因组织学异质性、侵袭能力强、术后复发快等问题,致使患者经手术治疗、化疗和放疗后的预后差,总体生存期短。GBM细胞来源外泌体(GBM cell-derived exosome,GBM-exo)能够通过其携带的细胞因子、miRNA、DNA和蛋白质等调节GBM细胞的增殖和迁移,通过血管生成蛋白和非编码RNA促进肿瘤血管生成,通过调节因子、蛋白质和药物靶向免疫检查点等介导肿瘤免疫逃逸,以及通过非编码RNA对抗GBM细胞的耐药性,有望成为个性化治疗GBM的重要靶标,且可以作为GBM的诊断和预后标志物。本文阐述了GBM-exo的制备方法和生物学特征,及其在GBM细胞增殖、血管生成、免疫逃逸和耐药性方面的作用和分子机制,为研发诊治GBM的新策略提供参考。Patients with glioblastoma(GBM)generally have a bad prognosis and short overall survival after being treated with surgery,chemotherapy or radiotherapy due to the histological heterogeneity,strong invasive ability and rapid postoperative recurrence of GBM.The components of GBM cell-derived exosome(GBM-exo)can regulate the proliferation and migration of GBM cell via cytokines,miRNAs,DNA molecules and proteins,promote the angiogenesis via angiogenic proteins and non-coding RNAs,mediate tumor immune evasion by targeting immune checkpoints with regulatory factors,proteins and drugs,and reduce drug resistance of GBM cells through non-coding RNAs.GBM-exo is expected to be an important target for the personalized treatment of GBM and a marker for diagnosis and prognosis of this kind of disease.This review summarizes the preparation methods,biological characteristics,functions and molecular mechanisms of GBM-exo on cell proliferation,angiogenesis,immune evasion and drug resistance of GBM to facilitate developing new strategies for the diagnosis and treatment of GBM.

关 键 词：胶质母细胞瘤 外泌体 细胞增殖 血管生成 免疫逃逸 耐药性 

分 类 号：R739.4[医药卫生—肿瘤]