In vivo tumor ultrasound-switchable fluorescence imaging via intravenous injections of size-controlled thermosensitive nanoparticles  

作　　者：Liqin Ren Yang Liu Tingfeng Yao Kytai TNguyen Baohong Yuan 

机构地区：[1]Ultrasound and Optical Imaging Laboratory,Department of Bioengineering,the University of Texas at Arlington,Arlington,TX 76019,USA [2]Joint Biomedical Engineering Program,the University of Texas at Arlington and the University of Texas Southwestern Medical Center,Dallas,TX 75390,USA [3]Department of Bioengineering,the University of Texas at Arlington,Arlington,TX 76019,USA

出　　处：《Nano Research》2023年第1期1009-1020,共12页纳米研究（英文版）

基　　金：This work was supported in part by funding from the National Institute of Biomedical Imaging and Bioengineering(No.1R15EB030809-01);the Research Enhancement Program(No.270089);the Cancer Prevention&Research Institute of Texas(Nos.RP170564 and RP210206).

摘　　要：Near-infrared fluorescence imaging has emerged as a noninvasive,inexpensive,and ionizing-radiation-free monitoring tool for assessing tumor growth and treatment efficacy.In particular,ultrasound switchable fluorescence(USF)imaging has been explored with improved imaging sensitivity and spatial resolution in centimeter-deep tissues.This study achieved the size control of polymer-based and indocyanine green(ICG)encapsulated USF contrast agents,capable of accumulating in the tumor after intravenous injections.These nanoprobes varied in size from 58 to 321 nm.The bioimaging profiles demonstrated that the proposed nanoparticles can efficiently eliminate the background light from normal tissue and show a tumor-specific fluorescence enhancement in the BxPC-3 tumor-bearing mice models possibly via the enhanced permeability and retention effect.In vivo tumor USF imaging further demonstrated that these nanoprobes can effectively be switched“ON”with enhanced fluorescence in response to a focused ultrasound stimulation in the tumor microenvironment,contributing to the high-resolution USF images.Therefore,our findings suggest that ICG-encapsulated nanoparticles are good candidates for USF imaging of tumors in live animals,indicating their great potential in optical tumor imaging in deep tissue.

关 键 词：in vivo tumor imaging deep tissue near-infrared(NIR)imaging high resolution ultrasound-switchable fluorescence imaging indocyanine green(ICG) 

分 类 号：R730[医药卫生—肿瘤] O657.3[医药卫生—临床医学]