Optical control of neuronal activities with photoswitchable nanovesicles  

作　　者：Hejian Xiong Kevin AAlberto Jonghae Youn Jaume Taura Johannes Morstein Xiuying Li Yang Wang Dirk Trauner Paul A.Slesinger Steven O.Nielsen Zhenpeng Qin 

机构地区：[1]Department of Mechanical Engineering,The University of Texas at Dallas,Richardson,TX 75080,USA [2]Department of Chemistry and Biochemistry,The University of Texas at Dallas,Richardson,TX 75080,USA [3]Nash Family Department of Neuroscience,Icahn School of Medicine at Mount Sinai,New York,NY 10029,USA [4]Department of Chemistry,New York University,New York,NY 10012,USA [5]Department of Bioengineering,The University of Texas at Dallas,Richardson,TX 75080,USA [6]Department of Surgery,The University of Texas at Southwestern Medical Center,Dallas,TX 75080,USA [7]Center for Advanced Pain Studies,The University of Texas at Dallas,Richardson,TX 75080,USA

出　　处：《Nano Research》2023年第1期1033-1041,共9页纳米研究（英文版）

基　　金：This work was partially supported by National Science Foundation under award number 2123971(Z.Q.,P.A.S.,and S.O.N.);National Institute of Neurological Disorders and Stroke of the National Institutes of Health under award number RF1NS110499(Z.Q.and P.A.S.);a postdoc research grant from the Phospholipid Research Center(Heidelberg,Germany)to H.X.

摘　　要：Precise modulation of neuronal activity by neuroactive molecules is essential for understanding brain circuits and behavior.However,tools for highly controllable molecular release are lacking.Here,we developed a photoswitchable nanovesicle with azobenzene-containing phosphatidylcholine(azo-PC),coined‘azosome’,for neuromodulation.Irradiation with 365 nm light triggers the trans-to-cis isomerization of azo-PC,resulting in a disordered lipid bilayer with decreased thickness and cargo release.Irradiation with 455 nm light induces reverse isomerization and switches the release off.Real-time fluorescence imaging shows controllable and repeatable cargo release within seconds(<3 s).Importantly,we demonstrate that SKF-81297,a dopamine D1-receptor agonist,can be repeatedly released from the azosome to activate cultures of primary striatal neurons.Azosome shows promise for precise optical control over the molecular release and can be a valuable tool for molecular neuroscience studies.

关 键 词：AZOBENZENE PHOTOSWITCH LIPOSOME controlled release NEUROMODULATION 

分 类 号：O43[机械工程—光学工程]