生长分化因子15在多发性骨髓瘤细胞MM.1R中的表达及生物学作用研究  

Expression and biological role of growth differentiation factor 15 in multiple myeloma cells of MM.1R

作　　者：吴菁 王禹 覃宏平 王其财 黄春妮[1] 姚奕斌[3] 凌志安[4] 李若林[2] WU Jing;WANG Yu;QIN Hongping;WANG Qicai;HUANG Chunni;YAO Yibin;LING Zhian;LI Ruolin(Department of Laboratory Medicine,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Clinical Medical Experiment Center,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Hematology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Orthopedics,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学第一附属医院检验科,广西南宁530021 [2]广西医科大学第一附属医院临床医学实验中心,广西南宁530021 [3]广西医科大学第一附属医院血液内科,广西南宁530021 [4]广西医科大学第一附属医院骨科,广西南宁530021

出　　处：《右江医学》2023年第4期307-313,共7页Chinese Youjiang Medical Journal

基　　金：广西自然科学基金(2020GXNSFAA297052);广西医疗卫生适宜技术开发与推广应用项目(S2018076);广西壮族自治区卫生健康委员会自筹经费科研课题(Z-A20220561)。

摘　　要：目的探讨生长分化因子15(growth differentiation factor 15,GDF15)在多发性骨髓瘤(multiple myeloma,MM)中的表达,并初步分析其对细胞生物学功能的影响。方法应用实时荧光定量PCR(qRT-PCR)检测GDF15在多发性骨髓瘤细胞株MM.1R中的表达水平。再通过构建GDF15的过表达和沉默质粒分别转染MM.1R细胞株,检测细胞增殖、细胞迁移、细胞侵袭、细胞周期和凋亡。结果在72 h、96 h、120 h三个时间点,过表达GDF15组细胞增殖较阴性对照组明显增高,差异有统计学意义(P均<0.05);过表达GDF15后MM.1R细胞的迁移和侵袭能力有所增加,但差异无统计学意义(P>0.05)。在72 h和96 h,沉默GDF15组细胞增殖较阴性对照组明显降低,差异有统计学意义(P<0.05);与阴性对照组相比,沉默GDF15组能显著抑制MM.1R细胞的迁移和侵袭能力。48 h后,沉默组G0/G1期细胞比例显著高于阴性对照组,沉默组S期细胞比例显著低于阴性对照组,差异均有统计学意义(P均<0.05),但未见明显诱导MM.1R细胞凋亡的作用。结论GDF15基因影响多发性骨髓瘤细胞MM.1R的增殖、迁移和侵袭能力,并调节细胞周期,这可能会促进多发性骨髓瘤的进展。Objective To investigate the expression of growth differentiation factor 15(GDF15)in multiple myeloma(MM),and to preliminarily analyze its effect on cell biological function.Methods The expression level of GDF15 in multiple myeloma cell line MM.1R was detected by quantitative real-time PCR(qRT-PCR).The MM.1R cell lines were then transfected by constructing overexpression and silent plasmids of GDF15,and cell proliferation,cell migration,cell invasion,cell cycle and apoptosis were detected.Results At 72 h,96 h and 120 h,cell proliferation in overexpressed GDF15 group was significantly higher than that in negative control group,and difference was statistically significant(all P<0.05);the migration and invasion capacity of MM.1R cells were increased with the GDF15 overexpression,but the difference was not statistically significant(P>0.05).At 72 h and 96 h,cell proliferation in silence GDF15 group was significantly lower than that in the negative control group,and the difference was statistically significant(P<0.05);compared with the negative control group,the silence GDF15 group significantly inhibited the migration and invasion of MM.1R cells.After 48 h,the proportion of cells in G0/G1 phase in the silence group was significantly higher than that in the negative control group,and the proportion of cells in S phase in the silence group was significantly lower than that in the negative control group,the differences were statistically significant(all P<0.05),but there was no obvious effect on inducing apoptosis of MM.1R cells.Conclusion GDF15 gene affects the proliferation,migration,and invasion ability of multiple myeloma cells MM.1R,and regulates cell cycle,which may promote the progression of multiple myeloma.

关 键 词：多发性骨髓瘤 生长分化因子15(GDF15) 生物学行为 细胞增殖 细胞迁移 

分 类 号：R733.3[医药卫生—肿瘤]