Methods of DNA introduction for the engineering of commensal microbes  

作　　者：Dake Liu Nicole E.Siguenza Amir Zarrinpar Yousong Ding 

机构地区：[1]Department of Medicinal Chemistry,Center for Natural Products,Drug Discovery and Development,University of Florida,Gainesville 32610,Florida,United States [2]Division of Gastroenterology,Center for Microbiome Innovation,University of California,La Jolla,San Diego 92093,California,United States [3]VA San Diego Health System,La Jolla 92161,California,United States

出　　处：《Engineering Microbiology》2022年第4期45-53,共9页工程微生物学（英文）

基　　金：supported by the National Institutes of Health U01 CA265719(to Y.D.and A.Z.);A.Z.is further supported by R01 EB030134 and received institutional support from NIH P30 DK120515,P30 DK063491,P30 CA014195,P50 AA011999 and UL1 TR001442;N.S.is a Biolegend Fellow and is supported by the National Science Foundation Graduate Research Fellowship Program under Grant No.1000340660。

摘　　要：The microbiome is an essential component of ecological systems and is comprised of a diverse array of microbes.Over the past decades,the accumulated observational evidence reveals a close correlation between the micro-biome and human health and disease.Many groups are now manipulating individual microbial strains,species and the community as a whole to gain a mechanistic understanding of the functions of the microbiome.Here,we discuss three major approaches for introducing DNA to engineer model bacteria and isolated undomesticated bacteria,including transformation,transduction,and conjugation.We provide an overview of these approaches and describe the advantages and limitations of each method.In addition,we highlight examples of human mi-crobiome engineering using these approaches.Finally,we provide perspectives for the future of microbiome engineering.

关 键 词：Genetic engineering TRANSFORMATION TRANSDUCTION CONJUGATION MICROBIOME Undomesticated microbes Therapeutic applications 

分 类 号：Q815[生物学—生物工程]