Single-cell RNA-sequencing reveals radiochemotherapy-induced innate immune activation and MHC-II upregulation in cervical cancer  

作　　者：Chao Liu Xiaohui Li Qingyu Huang Min Zhang Tianyu Lei Fuhao Wang Wenxue Zou Rui Huang Xiaoyu Hu Cong Wang Xiaoling Zhang Bing Sun Ligang Xing Jinbo Yue Jinming Yu 

机构地区：[1]Department of Radiation Oncology and Shandong Provincial Key Laboratory of Radiation Oncology,Shandong Cancer Hospital and Institute,Shandong First Medical University and Shandong Academy of Medical Sciences,Jinan 250117,China [2]Research Unit of Radiation Oncology,Chinese Academy of Medical Sciences,Jinan 250117,China [3]Department of Nephrology,First Medical Center of Chinese PLA General Hospital,Beijing 100853,China [4]Department of Oncology,Renmin Hospital of Wuhan University,Wuhan 430060,China [5]School of Clinical Medicine,Weifang Medical University,Weifang 261053,China [6]Department of Gynecologic Oncology,Shandong Cancer Hospital and Institute,Shandong First Medical University and Shandong Academy of Medical Sciences,Jinan 250117,China [7]Department of Radiation Oncology,Fifth Medical Center of Chinese PLA General Hospital,Beijing 100071,China

出　　处：《Signal Transduction and Targeted Therapy》2023年第2期827-841,共15页信号转导与靶向治疗（英文）

基　　金：This study was supported by funds from the National Natural Science Foundation of China(82030082 and 81871895);the Academic Promotion Program of Shandong First Medical University(2019ZL002);Key Research and Development Project of Shandong Province of China(2021CXGC011102);the Radiation Oncology Innovate Unit,Chinese Academy of Medical Sciences(2019RU071);Young Taishan Scholars and Academic Promotion Program of Shandong First Medical University(2019RC003);Shandong Provincial Natural Science Foundation(ZR2021QH006);Beijing Bethune Charitable Foundation(flzh202118).

摘　　要：Radiochemotherapy(RCT)is a powerful treatment for cervical cancer,which affects not only malignant cells but also the immune and stromal compartments of the tumor.Understanding the remodeling of the local ecosystem induced by RCT would provide valuable insights into improving treatment strategies for cervical cancer.In this study,we applied single-cell RNA-sequencing to paired pre-and post-RCT tumor biopsies from patients with cervical cancer and adjacent normal cervical tissues.We found that the residual population of epithelial cells post-RCT showed upregulated expression of MHC class II genes.Moreover,RCT led to the accumulation of monocytic myeloid-derived suppressor cells with increased pro-inflammatory features and CD16+NK cells with a higher cytotoxic gene expression signature.However,subclusters of T cells showed no significant increase in the expression of cytotoxic features post-RCT.These results reveal the complex responses of the tumor ecosystem to RCT,providing evidence of activation of innate immunity and MHC-II upregulation in cervical cancer.

关 键 词：cervical chemotherapy immunity 

分 类 号：R737.33[医药卫生—肿瘤]