三阴性乳腺癌组织中NRP-1和PD-L1表达及临床意义  

Expression and clinical significance of NRP-1 and PD-L1 in triple negative breast cancer

作　　者：马啸 刘浩楠 吕帝 崔永征 韩正祥 Ma Xiao;Liu Haonan;Lyu Di;Cui Yongzheng;Han Zhengxiang(Department of Oncology,The Affiliated Hospital of Xuzhou Medical University,Jiangsu Province,Xuzhou 221000,China)

机构地区：[1]徐州医科大学附属医院肿瘤科,江苏徐州221000

出　　处：《疑难病杂志》2023年第5期458-463,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：江苏省高层次卫生人才“六个一工程”A类项目(LGY2020006)。

摘　　要：目的分析三阴性乳腺癌(TNBC)组织中神经纤毛蛋白-1(NRP-1)和程序性死亡配体-1(PD-L1)的表达水平及其相关性和临床意义。方法收集2012年3月—2014年12月行手术治疗的TNBC患者40例的癌组织及其中10例对应的癌旁组织芯片,使用免疫组织化学法检测癌组织及癌旁组织中NRP-1和PD-L1表达水平,并分析NRP-1、PD-L1在不同临床病理特征间的表达差异;Spearman等级法分析两者的相关性;Kaplan-Meier法绘制生存曲线,多因素Cox回归模型分析TNBC患者预后相关影响因素。结果TNBC癌组织NRP-1、PD-L1蛋白表达水平高于癌旁组织(P<0.01);NRP-1的表达与肿瘤直径、病理分级明显相关(χ^(2)/P=6.218/0.013,4.392/0.036)。PD-L1的表达与病理分级、Ki67水平明显相关(χ^(2)/P=6.465/0.011,8.102/0.004)。NRP-1与PD-L1的表达呈正相关(r=0.570,P<0.001)。NRP-1、PD-L1高表达患者中位总生存期分别低于低表达患者(χ^(2)/P=14.510/0.002,9.641/0.001),NRP-1、PD-L1均高表达患者中位总生存期显著低于NRP-1、PD-L1均低表达患者(χ^(2)=15.940,P=0.001)。Cox回归分析显示,PD-L1高表达是患者预后的独立危险因素[HR(95%CI)=6.323(1.324~30.202),P=0.021]。结论NRP-1、PD-L1的表达可能与TNBC的发生、发展、预后有关,有望成为TNBC治疗的新靶点,联合NRP-1和PD-L1可用来评估TNBC的严重程度。Objective To analyze the expression levels of neurocilin-1(NRP-1)and programmed death ligand 1(PD-L1)in triple negative breast cancer(TNBC)tissues and their correlation and clinical significance.Methods Collect 40 cancer tissues and 10 corresponding adjacent tissues of TNBC patients who underwent surgical treatment at the Oncology Department of Xuzhou Medical University Affiliated Hospital from March 2012 to December 2014.Immunohistochemical methods were used to detect the expression levels of NRP-1 and PD-L1 in the cancer tissues and adjacent tissues,and the expression differences of NRP-1 and PD-L1 among different clinical and pathological features were analyzed;The Spearman hierarchy method was used to analyze the correlation between the two;Kaplan Meier method was used to draw survival curves,and a multivariate Cox regression model was used to analyze the prognostic factors related to TNBC patients.Results The expression levels of NRP-1 and PD-L1 proteins in TNBC cancer tissue were higher than those in adjacent tissues(P<0.01);The expression of NRP-1 is significantly correlated with tumor diameter and pathological grading(χ^(2)/P=6.218/0.013,4.392/0.036).The expression of PD-L1 are significantly correlated with pathological grading ki67 levels(χ^(2)/P=6.465/0.011,8.102/0.004).There is a positive correlation between the expression of NRP-1 and PD-L1(r=0.570,P<0.001).The median overall survival time of patients with high expression of NRP-1 and PD-L1 is lower than that of patients with low expression,respectively(χ^(2)/P=14.510/0.002,9.641/0.001),the median overall survival of patients with high expression of NRP-1 and PD-L1 was significantly lower than that of patients with low expression of NRP-1 and PD-L1(χ^(2)=15.940,P=0.001).Cox regression analysis showed that high expression of PD-L1 was an independent risk factor for patient prognosis[HR(95%CI)=6.323(1.324-30.202),P=0.021].Conclusion The expression of NRP-1 and PD-L1 may be related to the occurrence,development,and prognosis of TNBC,and may become a

关 键 词：三阴性乳腺癌 神经纤毛蛋白-1 程序性死亡配体-1 临床意义 

分 类 号：R737.9[医药卫生—肿瘤]