膀胱癌组织miR-20a-5p、NR4A3表达及其与患者临床病理特征、预后的关系  

Expression of miR-20a-5p and NR4A3 in bladder cancer and their relationship with clinicopathological characteristics and prognosis

作　　者：杨海北 王卫生 桂俊卿 徐跃文[1] 崔茂荣[1] 张超[1] 刘志明[1] Yang Haibei;Wang Weisheng;Gui Junqing;Xu Yuewen;Cui Maorong;Zhang Chao;Liu Zhiming(Department of Urology Surgery,the First People’s Hospital of Qujing,Yunnan Province,Qujing 655000,China)

机构地区：[1]云南省曲靖市第一人民医院泌尿外科,655000

出　　处：《疑难病杂志》2023年第5期464-468,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：云南省科技厅科技计划项目(202201AY070001-213)。

摘　　要：目的分析膀胱癌(BC)组织miR-20a-5p、核受体亚家族4成员3(NR4A3)表达及其与患者临床病理特征、预后的关系。方法选取2016年12月—2019年1月曲靖市第一人民医院泌尿外科行手术治疗BC患者30例,收集其膀胱癌组织及相应的癌旁组织。实时荧光定量PCR(qRT-PCR)检测BC癌组织中miR-20a-5p、NR4A3的表达水平,并分析miR-20a-5p、NR4A3表达与BC临床病理特征的关系;Kaplan-Meier曲线分析miR-20a-5p、NR4A3与BC患者预后的关系;Cox风险比例回归模型评估BC患者预后的影响因素。结果与癌旁组织比较,BC癌组织中miR-20a-5p表达上升(t/P=7.013/<0.001),NR4A3表达下降(t/P=9.658/<0.001)。TargetScan网站显示,miR-20a-5p与NR4A3存在靶向关系,且Pearson相关性分析显示,BC癌组织中miR-20a-5p与NR4A3表达呈负相关(r/P=-0.599/<0.001)。病理Ⅲ级、TNMⅢ~Ⅳ期、有淋巴结转移BC患者miR-20a-5p高于病理Ⅰ~Ⅱ级、TNMⅠ~Ⅱ期、无淋巴结转移者(t/P=2.159/0.040、2.157/0.040、2.403/0.023),病理Ⅲ级、TNMⅢ~Ⅳ期、有淋巴结转移BC患者NR4A3表达显著低于病理Ⅰ~Ⅱ级、TNMⅠ~Ⅱ期、无淋巴结转移者,差异均有统计学意义(t/P=3.421/0.002、5.445/<0.001、2.849/0.008)。BC患者3年总生存率为63.33%,miR-20a-5p高表达组3年生存率低于低表达组(χ^(2)/P=4.474/0.034),而NR4A3低表达组低于高表达组(χ^(2)/P=9.853/0.002);miR-20a-5p高表达是影响BC患者预后的独立危险因素[OR(95%CI)=2.028(1.764~2.895)]。结论BC癌组织中miR-20a-5p表达上升,NR4A3表达下降,与BC病理分级、淋巴结转移、TNM分期及预后有关,且miR-20a-5p高表达为BC患者预后的独立危险因素。Objective To analyze the expression of miR-20a-5p and nuclear receptor subfamily 4 member 3(NR4A3)in bladder cancer(BC)and their relationship with clinicopathological characteristics and prognosis.Methods From December 2016 to January 2019,30 patients with BC were selected from the Urology Department of the First People's Hospital of Qujing City for surgical treatment and their bladder cancer tissues and corresponding paracancerous tissues were collected.Real time fluorescence quantitative PCR(qRT-PCR)was used to detect the expression levels of miR-20a-5p and NR4A3 in BC cancer tissue,and to analyze the relationship between the expression of miR-20a-5p and NR4A3 and the clinical pathological characteristics of BC;Kaplan-Meier curve analysis of the relationship between miR-20a-5p,NR4A3 and the prognosis of BC patients;The Cox risk proportional regression model evaluates the influencing factors of prognosis in BC patients.Results Compared with adjacent tissues,the expression of miR-20a-5p in BC cancer tissue increased(t/P=7.013/<0.001),while the expression of NR4A3 decreased(t/P=9.658/<0.001).TargetScan website shows that there is a targeted relationship between miR-20a-5p and NR4A3,and Pearson correlation analysis shows a negative correlation between miR-20a-5p and NR4A3 expression in BC cancer tissue(r/P=-0.599/<0.001).Patients with pathological grade III,TNM stage III-IV,and BC without lymph node metastasis had higher miR-20a-5p than those with pathological grade I-II,TNM stage I-II,and lymph node metastasis(t/P=2.159/0.040,2.157/0.040,2.403/0.023).Patients with pathological grade III,TNM stage III-IV,and BC with lymph node metastasis had lower NR4A3 expression than those with pathological grade I-II,TNM stage I-II,and BC without lymph node metastasis,the differences were statistically significant(t/P=3.421/0.002,5.445/<0.001,2.849/0.008).The overall 3-year survival rate of BC patients is 63.33%,and the 3-year survival rate of the miR-20a-5p high expression group is lower than that of the low expression group(χ

关 键 词：膀胱癌 miR-20a-5p 核受体亚家族4成员3 预后 

分 类 号：R737.14[医药卫生—肿瘤]