高效毛细管电泳法测定金针菇提取物中β-烟酰胺单核苷酸含量的初步研究  

Preliminary Study on Determination ofβ-nicotinamide Mononucleotide Content from Flammulina velutiper Extract by High Performance Capillary Electrophoresis

作　　者：马晓颖[1] 肖军[1] 陈珣[1] 龚娜[1] 刘国丽[1] 杨涛[1] 肇莹[1] MA Xiaoying;XIAO Jun;CHEN Xun;GONG Na;LIU Guoli;YANG Tao;ZHAO Ying(Institute of Edible Mushrooms,Liaoning Academy of Agricultural Sciences,Key Laboratory of Quality Cultivation of Edible Mushrooms in Liaoning Province,Shenyang 110161,China)

机构地区：[1]辽宁省农业科学院食用菌研究所,辽宁省食用菌优质栽培重点实验室,沈阳110161

出　　处：《中国农业科技导报》2023年第2期220-226,共7页Journal of Agricultural Science and Technology

基　　金：揭榜挂帅科技攻关专项(2021JH1/10400035);辽宁农业攻关及产业化项目(2020JH2/10200013);中央引导地方科技发展专项资金(2021010053-JH6/105);辽宁科技特派行动专项计划(2021JH5/10400035);沈阳市农村科技特派团专项(21-116-3-23);辽宁省农业科学院院长基金项目(2022QN2303)。

摘　　要：金针菇既是一种美味食品,又是较好的保健食品,营养成分十分丰富,建立了金针菇提取液中β-烟酰胺单核苷酸(β-nicotinamide mononucleotide,NMN)的高效毛细管电泳(high performance capillary electrophoresis,HPCE-DAD)含量测定方法。试验结果表明,检测波长218 nm,运行缓冲液30 mmol·L^(-1)硼砂缓冲液和10 mmol·L^(-1)磷酸二氢钠缓冲液(pH 9.2),分离电压25 kV,进样时间为5 s时,该方法能够检测到金针菇提取液中的β-烟酰胺单核苷酸,检测限为0.17μg·mL^(-1),并在0.03~2.00 mg·mL^(-1)范围内呈较好的线性关系,利用标准曲线获得回归方程y=2E+6x-345.69,R2=0.9994,将金针菇图谱中的相应β-烟酰胺单核苷酸(NMN)峰面积带入回归方程,计算得β-烟酰胺单核苷酸含量为27.57μg·mL^(-1)。方法学考察结果表明β-烟酰胺单核苷酸标准品迁移时间和峰面积相对偏差为0.68%和1.44%;且样品8 h内的迁移时间和峰面积的相对偏差分别为1.26%和2.82%;β-烟酰胺单核苷酸在金针菇样品中的回收率为89%~94%。结果表明该方法适用于金针菇提取液中β-烟酰胺单核苷酸的测定,为食用菌中β-烟酰胺单核苷酸含量的测定提供了新的方法。Flammulina velutiper is not only a delicious food,but also a good health food which is rich in nutrition.However,there was no report on the determination ofβ-nicotinamide mononucleotide(NMN)content in Flammulina velutiper.A method for the determination ofβ-nicotinamide mononucleotide(NMN)in Flammulina velutiper was established by high performance capillary electrophoresis(HPCE-DAD).The detection conditions included detection wavelength 218 nm;running buffer(30 mmol·L^(-1)boron sand buffer pH 9.2;10 mmol·L^(-1)phosphate bobbin);separation voltage of 25 kV;injection time of 5 s.The results showed that the method could detect theβ-nicotinamide mononucleotide content of fruits from Flammulina velutiper with the detection limit of 0.17μg·mL^(-1),and showed a good linear relationship from 0.03 to 2.00 mg·mL^(-1).The regression equation was obtained by the standard curve:y=2E+6x-345.69,R2=0.9994,the correspondingβ-nicotinamide mononucleotide(NMN)peak area was put into the regression equation,and the calculated content was 27.57μg·mL^(-1).The relative standard deviations of migration times forβ-nicotinamide mononucleotide and migration peak areas were 0.68%and 1.44%.The relative deviations of migration time and peak area within 8 h were 1.26%and 2.82%,respectively.The recoveries ofβ-nicotinamide mononucleotide in Flammulina velutiper were 89%~94%.These results indicated that the method was suitable for the determination ofβ-nicotinamide mononucleotide in Flammulina velutiper and provided a new method for the determination ofβ-nicotinamide mononucleotide in edible fungus.

关 键 词：高效毛细管电泳 β-烟酰胺单核苷酸 金针菇 

分 类 号：S646[农业科学—蔬菜学]