红景天苷预防给药对MCAO大鼠的神经保护作用  

The neuroprotective effect of salidroside prophylactic administration on MCAO model rats

作　　者：黄私迎 聂婧雯 罗锐 周彬彬 余正双 黄嘉慧 赖文芳[1] 洪桂祝 HUANG Si-ying;NIE Jing-wen;LUO Rui;ZHOU Bin-bin;YU Zheng-shuang;HUANG Jia-hui;LAI Wen-fang;HONG Gui-zhu(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中国药理学通报》2023年第5期890-896,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 82174001,81973503);福建省自然科学基金项目(No 2021J02014,2020J01727);福建省卫健委中青年骨干人才培养项目(No 2019-ZQN-74)。

摘　　要：目的研究红景天苷(salidroside,Sal)预防给药对MCAO大鼠的神经保护作用。方法SD成年雄性大鼠52只,随机分:假手术组(Sham)、模型组(MCAO)、红景天苷预给药组(MCAO+Sal)。Sal给药剂量为50 mg·kg^(-1)。连续腹腔注射给药7 d,d 7给药0.5 h后,线栓法制作MCAO模型,24 h后麻醉取材。TTC染色检测大鼠脑梗死体积,TUNEL染色测定神经细胞凋亡数,Western blot测定脑组织中Bax、Bcl-2、cleaved Caspase-3和总Caspase-3蛋白的表达,萘酚AS-D氯乙酸酯酶检测脑组织中性粒细胞的表达,RT-qPCR法分析IL-1β、IL-6、TNF-α的mRNA表达,免疫荧光法分析脑组织中NeuN的表达。结果Sal预给药7 d明显减少了MCAO大鼠脑梗死体积和TUNEL阳性细胞数,促进Bcl-2蛋白的表达,抑制Bax、cleaved Caspase-3蛋白的表达,但对总Caspase-3蛋白无明显作用,减少缺血侧脑组织中性粒细胞的募集,降低炎症因子IL-1β、IL-6、TNF-α的mRNA表达,上调NeuN的表达。结论Sal预给药7 d能明显降低MCAO大鼠脑梗死体积,抑制神经细胞凋亡,减轻炎症,促进NeuN的表达,进而起到神经保护作用。Aim To investigate the neuroprotective effect of prophylactic administration of salidroside(Sal)on MCAO rats.Methods A total of 52 SD adult male rats were randomly divided into sham operation group(Sham),model group(MCAO)and salidroside pre-administration group(MCAO+Sal).The dose of Sal was 50 mg·kg^(-1).MCAO model was administered by continuous intraperitoneal injection for 7 days,followed by the wire embolization 0.5 h after the 7th day of administration,and the materials were taken 24 h after anesthesia.TTC staining was used to detect the volume of cerebral infarction in rats,TUNEL staining was used to detect the number of nerve cell apoptosis,Western blot was used to detect the expression of Bax,Bcl-2,cleaved Caspase-3 and total Caspase-3 proteins,naphthol AS-D chloroacetate was used to detect the expression of neutrophils in brain tissues,RT-qPCR was used to detect the mRNA expression of IL-1β,IL-6 and TNF-α,and immunofluorescence staining was used to determine the expression of NeuN in brain tissues.Results Pre-administration of salidroside for seven days significantly reduced cerebral infarct volume and nerve cell apoptosis in MCAO rats,promoted the expression of Bcl-2 protein,inhibited the expression of Bax and cleaved Caspase-3 proteins,but it had no significant effect on total Caspase-3 protein,and it reduced the recruitment of neutrophils in ischemic brain tissues,decreased the mRNA expression of inflammatory factors IL-1β,IL-6,TNF-α,and up-regulated the expression of NeuN.Conclusions Pre-administration of salidroside for seven days can significantly reduce the volume of cerebral infarction in MCAO rats,inhibit nerve cell apoptosis,reduce inflammation and promote the expression of NeuN,then playing a neuroprotective role.

关 键 词：红景天苷 MCAO 神经保护 凋亡 抗炎 NEUN 

分 类 号：R-332[医药卫生] R284.1R322.81R329.25R392.12R743.3