抗FGFR2 IVIg对口腔鳞癌细胞的抑制作用  

Inhibitory effect of natural anti-FGFR2 IVIg antibodies on OSCC cells

作　　者：王钰 刘秀 李鸿宇 董蕊[2] 胡颖[1] WANG Yu;LIU Xiu;LI Hong-yu;DONG Rui;HU Ying(Beijing Institute of Dental Research,Beijing Stomatology Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学口腔医学院口腔医学研究所,北京100050 [2]首都医科大学口腔医学院教育处,北京100050

出　　处：《北京口腔医学》2023年第2期93-98,共6页Beijing Journal of Stomatology

基　　金：国家自然科学基金(82071103,81570958);北京市自然基金(7162075)。

摘　　要：目的 探讨抗FGFR2 IVIg天然抗体对口腔鳞癌细胞的影响。方法 qRT-PCR实验检测口腔鳞癌细胞CAL27和SCC25中相关基因表达。采用in-house ELISA方法从正常个体血浆中筛查富含和低含抗FGFR2 IgG天然抗体的血浆,分别制备成抗FGFR2静脉注射免疫球蛋白(IVIg)和普通IVIg。CCK-8实验、流式细胞术和Transwell实验检测抗FGFR2 IVIg对细胞增殖、凋亡和侵袭能力的影响以及补体途径的参与作用。Western Blot实验检测凋亡和侵袭相关因子的表达。结果 CAL27和SCC25细胞中,FGFR2显著高表达。抗FGFR2 IVIg可以显著抑制CAL27和SCC25细胞的增殖和侵袭,诱导细胞凋亡.并上调Caspase 9和Cleaved Caspase 3诱导细胞凋亡,下调N-Cadherin、Zeb和Snail抑制细胞侵袭。灭活培养液中阴性血浆内补体后,抗FGFR2 IVIg对细胞增殖的抑制作用消失。结论 抗FGFR2 IVIg有望成为治疗口腔鳞癌的低毒新型制剂。Objective To investigate the effect of natural anti-FGFR2 IVIg antibodies on oral squamous cell carcinoma(OSCC)cells.Methods qRT-PCR assay was utilized to determine the expression of pertinent genes in OSCC cells CAL27 and SCC25.The level of natural antibodies in healthy donors was screened using in-house ELISA and divided into anti-FGFR2 IgG-abundant plasma and anti-FGFR2 IgG-deficient plasma,and then prepared into anti-FGFR2 intravenous immunoglobulin(IVIg)and regular IVIg,respectively.CCK-8 assay,flow cytometry assay and Transwell assay were used to measure the impact of anti-FGFR2 IVIg on cell proliferation,apoptosis and invasion,as well as the effect of complement-mediated cytotoxic(CDC).Western Blot assay was used to examine the expression of factors linked to apoptosis and invasion.Results FGFR2 was substantially more expressed than other genes in CAL27 and SCC25.Anti-FGFR2 IVIg significantly inhibited the proliferation,invasion and induced apoptosis of CAL27 and SCC25 compared with the same concentration of regular IVIg.By up-regulating Caspase 9 and Cleaved Caspase 3,anti-FGFR2 IVIg could cause apoptosis,and down-regulating N-Cadherin,Zeb,and Snail inhibited invasion.After the complement was inactivated,the inhibitory effect of anti-FGFR2 IVIg on cell proliferation disappeared.Conclusions Anti-FGFR2 IVIg is expected to become a new low-toxicity agent for the treatment of oral squamous cell carcinoma.

关 键 词：天然抗体 FGFR2 口腔鳞癌 静脉注射免疫球蛋白 

分 类 号：R739.8[医药卫生—肿瘤]