云南省稻瘟病菌群体的致病性分析及交配型分布  

Pathogenicity and mating type of rice blast fungus Magnaporthe oryzae isolates in Yunnan Province

作　　者：董丽英[1] 刘树芳[1] 田维逵 周伍民 张先闻 李迅东[1] 杨勤忠[1] Dong Liying;Liu Shufang;Tian Weikui;Zhou Wumin;Zhang Xianwen;Li Xundong;Yang Qinzhong(Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province,Institute of Agricultural Environment and Resource Research,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan Province,China;Plant Protection and Quarantine Station of Menghai County,Yunnan,Menghai 666200,Yunnan Province,China;Institute of Resource Plants Research,Yunnan University,Kunming 650091,Yunnan Province,China)

机构地区：[1]云南省农业科学院农业环境资源研究所,云南省农业跨境有害生物绿色防控重点实验室,昆明650205 [2]云南省勐海县植保植检站,勐海666200 [3]云南大学资源植物研究院,昆明650091

出　　处：《植物保护学报》2023年第2期316-324,共9页Journal of Plant Protection

基　　金：国家自然科学基金(32260646);云南省农业联合专项(202101BD070001-067);云南省重大科技专项计划(202102AE090003)。

摘　　要：为明确云南省不同稻区稻瘟病菌Magnaporthe oryzae的毒性频率及交配型分布,利用2007—2013年分离自云南省不同稻区的112株稻瘟病菌单孢菌株,对23个持有不同抗性基因的单基因系和持有Pi57(t)的水稻渗入系IL-E1454进行致病性测定。结果表明,稻瘟病菌对不同抗病基因的毒性频率存在很大差异,分离自粳稻区的稻瘟病菌菌株对持有Piz-t、Pi5、Pi9、Pi20和Pi57(t)这5个水稻品系的毒性频率分别为14.29%、5.36%、5.51%、5.36%和0;分离自籼稻区的稻瘟病菌菌株对持有Pik-h、Piz、Pita、Piz-5、Pita-2、Pi5、Pi7和Pi9这8个水稻品系的毒性频率分别为18.25%、9.13%、9.64%、7.50%、15.72%、0、13.05%和0;分离自陆稻区的稻瘟病菌菌株菌株对持有Pik-h、Pib、Pish、Pi1、Pi5、Pi9、Pi11和Pi57(t)这8个水稻品系的毒性频率分别为6.67%、3.33%、13.79%、13.33%、7.69%、6.67%、0和3.23%;交配型测定结果显示,陆稻区菌株可交配率为100.00%,其中MAT1-1和MAT1-2菌株分别为43.33%和56.67%;粳稻区菌株可交配率为91.84%,其中MAT1-1和MAT1-2菌株分别为69.39%和22.45%;籼稻区菌株可交配率为87.88%,其中MAT1-1和MAT1-2菌株分别为18.18%和69.70%,表明不同稻区稻瘟病菌的育性均比较高,但交配型分布极不平衡。In order to understand the virulence frequency and mating type distribution of rice blast fungusMagnaporthe oryzae in different rice-growing regions of Yunnan Province,112 isolates of M.oryzae collected from different rice-growing regions of Yunnan from 2007 to 2013 were tested on 23 monogenic lines and the introgression line IL-E1454 carrying blast resistant gene Pi57(t).The results indicated that the frequency of M.oryzae isolates virulent to different rice lines showed significant differences.The frequency of M.oryzae isolates from japonica-rice-growing regions virulent to Piz-t,Pi5,Pi9,Pi20 and Pi57(t)were 14.29%,5.36%,5.51%,5.36%and 0,respectively;the frequency of M.oryzae isolates from indica-rice-growing regions virulent to Pik-h,Piz,Pita,Piz-5,Pita-2,Pi5,Pi7 and Pi9 were 18.25%,9.13%,9.64%,7.50%,15.72%,0,13.05%and 0,respectively;the frequency of M.oryzae isolates from upland-rice-growing regions virulent to Pik-h,Pib,Pish,Pi1,Pi5,Pi9,Pi11 and Pi57(t)were 6.67%,3.33%,13.79%,13.33%,7.69%,6.67%,0 and 3.23%,respectively.Determination of mating type with four standard tested isolates showed that all strains from upland rice were fertile,and MAT1-1 and MAT1-2 isolates accounted for 43.33%and 56.67%of total isolates,respectively;the percentage of fertile isolates from japonica rice-growing regions was 91.84%,and MAT1-1 and MAT1-2 isolates accounted for 69.39%and 22.45%,respectively;the percentage of fertile isolates from indica rice-growing regions accounted for 87.88%,of them MAT1-1 and MAT1-2 isolates accounted for 18.18%and 69.70%,respectively.The fertility of M.oryzae isolates was high in different rice areas,but the distribution of their mating types was uneven in some populations.

关 键 词：稻瘟病菌 致病性 毒性频率 交配型 云南省 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]