脆弱拟杆菌灭活菌在迟发型过敏反应中的作用  

The effect of inactivated Bacteroides fragilis on delayed-type hypersensitivity

作　　者：闫馨文 常秀娟 柳珂 刘洋洋 王晔 白杨[1] 智发朝[1] YAN Xin-wen;CHANG Xiu-juan;LIU Ke;LIU Yang-yang;WANG Ye;BAI Yang;ZHI Fa-chao(Guangdong Provincial Key Laboratory of Gastroenterology,Institute of Gastroenterology of Guangdong Province,Department of Gastroenterology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Guangzhou Zhiyi Biotechnology Co.Ltd.,Guangzhou 510530,China)

机构地区：[1]南方医科大学南方医院消化科,广东省南方消化病研究,广东省胃肠病重点实验室,510515 [2]广州知易生物科技有限公司,510530

出　　处：《现代消化及介入诊疗》2023年第2期177-180,186,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：广东省重点实验室项目(2017B030314037)。

摘　　要：目的拟探究脆弱拟杆菌灭活菌(IBF)在迟发型过敏反应(DTH)中的免疫调节作用。方法野生型BALB/c小鼠随机分为对照组、模型组、低(1×106 CFU/只)、中(1×108 CFU/只)、高(1×1010 CFU/只)剂量IBF组及左旋咪唑组(30 mg/kg),每组10只小鼠。在予环磷酰胺免疫抑制后,2,4-二硝基氟苯(DNFB)所诱导的DTH模型中研究IBF是否具有免疫调节能力。小鼠耳肿胀程度、胸腺指数、脾脏指数为主要的观察指标,同时对小鼠各类血细胞进行计数。结果中、高剂量IBF可明显增加受环磷酰胺抑制的小鼠耳廓肿胀,同时可调节白细胞、单核细胞、中性粒细胞、嗜碱性粒细胞、嗜酸性粒细胞数量。各剂量IBF对胸腺指数无明显改善,均可显著增加脾脏指数。结论IBF可恢复小鼠对外源性过敏原所导致的迟发性超敏反应,且能对抗环磷酰胺引起的脾脏萎缩。Objective We aim to explore the immunomodulatory effect of inactivated bacteroides fragilis(IBF)in delayed-type hypersensitivity(DTH).Methods Wild-type BALB/c mice were randomly divided into control group,model group,low(1×106 CFU),medium(1×108 CFU),high(1×1010 CFU)dose of IBF group and levamisole group,10 mice for each group respectively.After immunosuppression with cyclophosphamide(Cy),the DTH model induced by 2,4-dinitrofluorobenzene(DNFB)was used to study whether IBF has immunomodulatory ability.The degree of ear swelling,thymus index and spleen index were the main observation indicators.Various blood cells of mice were counted at the same time.Results The medium and high dose of IBF could significantly increase the ear swelling of mice inhibited by cyclophosphamide,and regulate the number of white blood cells,monocytes,neutrophils,basophils and eosinophils.Each dose of IBF has no significant improvement on thymus index,but can significantly increase spleen index.Conclusion IBF can recover the DTH caused by exogenous allergens in mice,and can resist the spleen atrophy caused by Cy.

关 键 词：益生菌 脆弱拟杆菌 迟发型过敏反应 

分 类 号：R57[医药卫生—消化系统]