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作 者:雷蕾[1] 李雨 卢林 杨墅冰 杨桦[3] LEI Lei;LI Yu;LU Lin;YANG Shubing;YANG Hua(The IT Electronics Eleventh Design&research Institute Science and Technology Engineering co,LTD,Chengdu 610021,China;The Seed Management Station of Ganzi Tibetan Autonomous Prefecture,Kangding 626000,China;College of Forestry,Sichuan Agricultural University,Chengdu 611130,China)
机构地区:[1]信息产业电子第十一设计研究院科技工程股份有限公司,四川成都610021 [2]甘孜州种子管理站,四川康定626000 [3]四川农业大学林学院,四川成都611130
出 处:《四川林业科技》2023年第2期38-43,共6页Journal of Sichuan Forestry Science and Technology
基 金:四川省重点研发项目(19ZDYF)。
摘 要:松墨天牛(Monochamus alternatus)是松材线虫(Bursaphelenchus xylophilus)传播的主要媒介昆虫之一,为了更有效地控制松材线虫病的传播,本文通过比较6株不同来源白僵菌对松墨天牛的毒力,筛选出致病性最好的菌株与3种高分子材料混合制备成微胶囊,比较不同微胶囊在川硬皮肿腿蜂(Scleroderma sichuanensis)体表的附着力以及室内对松墨天牛的侵染效果。结果表明:分离自松墨天牛感病虫体的菌株B2在生长性状和对松墨天牛的致病力上均优于其他菌株;海藻酸钠微胶囊对川硬皮肿腿蜂体表附着率达63%以及室内试验中对松墨天牛的侵染率达60%。本次研究证明了利用天敌昆虫携带病原真菌微胶囊防治蛀干害虫的可行性。Monochamus alternatus is one of the main vector insects transmitted by Bursaphelenchus xylophilus.In order to control the transmission of pine wood nematode disease more effectively,the virulence of 6 strains of Beauveria bassiana from different sources to Monochamus alternatus was compared,and the strains with the best pathogenicity were selected and mixed with 3 kinds of polymer materials to prepare microcapsules.The adhesion of different microcapsules on the body surface of scleroderma sichuanensis and the indoor infection effect on Monochamus alternatus were compared,and the Beauveria bassiana microcapsules with the best infection effect were applied to the forest control experiment.The results showed that strain B2 isolated from the susceptible body of Monochamus alternatus was superior to other strains in growth characters and pathogenicity to Monochamus alternatus.The surface adhesion rate of sodium alginate microcapsule to scleroderma sichuanensis was 63%,and the infection rate of Monochamus alternatus was 60%in laboratory test.This study proved the feasibility of using natural enemy insects to carry pathogenic fungi microcapsules to control stem borers.rol stem borers.
关 键 词:白僵菌 高分子材料 微胶囊 川硬皮肿腿蜂 松墨天牛
分 类 号:S763.18[农业科学—森林保护学]
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