High temperature increases centromeremediated genome elimination frequency and enhances haploid induction in Arabidopsis  

作　　者：Ulkar Ahmadli Manikandan Kalidass Lucie Crhak Khaitova Joerg Fuchs Maria Cuacos Dmitri Demidov Sheng Zuo Jana Pecinkova Martin Mascher Mathieu Ingouff Stefan Heckmann Andreas Houben Karel Riha Inna Lermontova 

机构地区：[1]Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstrasse 3,06466 Seeland,Germany [2]Central European Institute of Technology(CEITEC)and National Centre for Biomolecular Research,Faculty of Science,Masaryk University,Kamenice 5,62500 Brno,Czech Republic [3]CIRAD,DIADE,IRD,University of Montpellier,34393 Montpellier,France

出　　处：《Plant Communications》2023年第3期126-138,共13页植物通讯（英文）

基　　金：supported by the German Federal Ministry of Education and Research(Plant 2030,Project 031B0192NN,HaploTools);the Deutsche Forschungsgemeinschaft(LE2299/3-1 and LE2299/5-1);the European Regional Development Fund-Project"REMAP"(CZ.02.1.01/0.0/0.0/15_003/0000479)to K.R.

摘　　要：Double haploid production is the most effective way to create true-breeding lines in a single generation.In Arabidopsis,haploid induction via mutation of the centromere-specific histone H3(cenH3)has been shown when the mutant is outcrossed to the wild-type,and the wild-type genome remains in the haploid progeny.However,factors that affect haploid induction are still poorly understood.Here,we report that a mutant of the cenH3 assembly factor Kinetochore Null2(KNL2)can be used as a haploid inducer when pollinated by the wild-type.We discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction 10-fold.We also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines,suggesting that haploidinducing lines in crops can be identified in a naturally occurring or chemically induced mutant population,avoiding the generic modification(GM)approach at any stage.Furthermore,a cenh3-4 mutant functioned as a haploid inducer in response to short-term heat stress,even though it did not induce haploids under standard conditions.Thus,we identified KNL2 as a new target gene for the generation of haploid-inducer lines and showed that exposure of centromeric protein mutants to high temperature strongly increases their haploid induction efficiency.

关 键 词：CENTROMERE kinetochore null 2 CENPC-k cenh3-4 temperature stress haploid induction 

分 类 号：Q94[生物学—植物学]