大豆种质耐碱性状全基因组关联分析  

Genome-wide association analysis of alkali tolerance traits in soybean germplasm

作　　者：滕卫丽[1] 史飞飞 林峰 刘晨煦 高鹏[1] 赵慧艳 赵雪[1] 韩英鹏[1] 李文滨[1] TENG Weili;SHI Feifei;LIN Feng;LIU Chenxu;GAO Peng;ZHAO Huiyan;ZHAO Xue;HAN Yingpeng;LI Wenbin(Northeast Agricultural University,Key Laboratory of Soybean Biology in Chinese of Ministry Education,Key Laboratory of Soybean Biology and Breeding(Genetics)of Ministry of Agriculture and Rural Affairs,Harbin 150030,China)

机构地区：[1]东北农业大学,大豆生物学教育部重点实验室,农业农村部东北大豆生物学与遗传育种重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2023年第4期1-13,共13页Journal of Northeast Agricultural University

基　　金：国家重点研发计划项目(2021YFD1201103-01-03);财政部、农业农村部现代农业产业技术体系建设专项资金项目(CARS-04-PS04)。

摘　　要：以255份大豆种质为试验材料,利用混合碱模拟大庆市苏打盐碱地碱胁迫环境,在大豆种质芽期和苗期分别调查耐碱相关性状,结合BLINK和FarmCPU两种方法进行全基因组关联分析。结果表明,共检测到64个关联SNP位点,其中3:37513903、3:37490361、9:37033917等7个SNP位点相对稳定,可靠性较高;筛选到26个功能注释基因,根据功能注释和基因表达量,推测3个基因与耐碱相关,分别为Glyma.03G160200、Glyma.09G149900和Glyma.19G067400。In this study,255 soybean germplasm were used as experimental materials,the mixed alkali was used to simulate the alkali tolerance environment of soda saline-alkali land in Daqing City.The alkali tolerance related traits were investigated at germination and seedling stages of soybean germplasm.BLINK and FarmCPU were used for genome-wide association analysis.The results showed that a total of 64 associated SNP loci were detected by the two methods,of which seven loci such as 3:37513903,3:37490361 and 9:37033917 were relatively stable SNP loci with high reliability.A total of 26 genes with functional annotation were screened out.According to functional annotation and gene expression level,three genes related to alkali tolerance were speculated,which were Glyma.03G160200,Glyma.09G149900 and Glyma.19G067400.

关 键 词：大豆 碱胁迫 芽期 苗期 全基因组关联分析 

分 类 号：S565.1[农业科学—作物学]