环境相关浓度双酚AF暴露对成年雌性弓背青鳉生长和生殖的毒性效应  

Toxic Effects of Bisphenol AF Exposure at Environmental Concentrations on the Growth and Reproduction of Adult Female Oryzias curvinotus

作　　者：黄泽胤 谢明花 陈月碧 黎学友 郭昱嵩[1] 王中铎[1,2] 董忠典 HUANG Ze-yin;XIE Ming-hua;CHEN Yue-bi(Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes/Fisheries College of Guangdong Ocean University,Zhanjiang,Guangdong 524088)

机构地区：[1]广东海洋大学水产学院/南海水产经济动物增养殖重点实验室,广东湛江524088 [2]湖南师范大学生命科学学院/省部共建淡水鱼类发育生物学国家重点实验室,湖南长沙410081

出　　处：《安徽农业科学》2023年第8期79-85,共7页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(41806195,31972794);大学生创新训练项目(580520041);广东海洋大学南海学者项目(QNXZ201807,201903);省部共建淡水鱼类发育生物学国家重点实验室开放课题(2020KF004)。

摘　　要：以成年雌性弓背青鳉(Oryzias curvinotus)为研究对象,研究了不同环境相关浓度(0.93、9.33和102.33μg/L)双酚AF(BPAF)短期暴露对其生存、生长和生殖的影响。结果表明,102.33μg/L BPAF暴露21 d极显著降低了成年雌性弓背青鳉的存活率和生长状况因子;9.33和102.33μg/L BPAF暴露导致成年雌性弓背青鳉肝细胞排列松散、脂肪积累减少,102.33μg/L BPAF暴露还导致肝细胞形态出现明显肿胀;102.33μg/L BPAF暴露导致其卵母细胞大多停留在初级卵母细胞阶段,抑制了卵母细胞的成熟。另外,102.33μg/L BPAF暴露显著或极显著上调了vtglike、vtg1、vtg2和chgh在肝脏中的转录水平以及cyp11a、cyp19a1a和gsdf在卵巢中的转录水平。不同环境相关浓度的BPAF暴露对成年弓背青鳉雌鱼有较强的致死效应、生长抑制效应、雌激素效应,并可引起肝损伤和卵母细胞发生障碍。Adult female Oryzias curvinotus were used as research objects to study the effects of short-term exposure with BPAF at different environmental concentrations(0.93,9.33 and 102.33μg/L)on the survival,growth and reproduction of O.curvinotus.The results showed that the survival rate and condition factor of adult female O.curvinotus were extremely significantly reduced by the exposure with 102.33μg/L BPAF for 21 days.Exposure with 9.33 and 102.33μg/L BPAF resulted in loose arrangement of hepatocytes and the reduction of fat accumulation in adult female O.curvinotus.Exposure with 102.33μg/L BPAF caused significant swelling of liver cell morphology.102.33μg/L BPAF caused oocytes to stay in the primary oocyte stage and inhibited the maturation of oocytes.102.33μg/L BPAF significantly or extremely significantly up-regulated the transcript levels of vtglike,vtg1,vtg2 and chgh in the liver,and the transcript levels of cyp11a,cyp19a1a and gsdf in the ovary.Exposure with environmental concentrations of BPAF had strong lethal effects,growth inhibitory effects,and estrogen effects on adult female O.curvinotus,and could cause liver damage and oocyte dysfunction.

关 键 词：弓背青鳉 内分泌干扰物 双酚AF 雌激素效应 生殖毒性 

分 类 号：X174[环境科学与工程—环境科学]