艾司氯胺酮对小鼠体外成骨细胞的影响  

Effects of esketamine on mouse osteoblasts in vitro

作　　者：崔荞荞 郭晓霞[1] 卢蒙恩 牛燕兰[2] 马智聪[2] CUI Qiaoqiao;GUO Xiaoxia;LU Meng’en;NIU Yanlan;MA Zhicong(Department of Anesthesiolgy,Second Affiliated Hospital,School of Anesthesiology,Shanxi Medical University,Taiyuan 030001,China;Department of Anesthesiology,Second Clinical Medical College,Shanxi Medical University)

机构地区：[1]山西医科大学麻醉学院附属第二医院麻醉教研室,太原030001 [2]山西医科大学第二临床医学院麻醉科

出　　处：《山西医科大学学报》2023年第3期358-363,共6页Journal of Shanxi Medical University

基　　金：山西省自然科学基金资助项目(201901D111368)。

摘　　要：目的观察艾司氯胺酮对小鼠体外成骨细胞的影响。方法选用4~6周C57BL雄性小鼠的后肢骨中的骨髓间充质干细胞(BMSCs),定向诱导分化为成骨细胞,采用CCK-8法筛选出对成骨细胞增殖无毒性的艾司氯胺酮浓度区间,后将定向诱导分化来的成骨细胞随机分为2组:空白组和艾司氯胺酮组(Ket组,100μmol/L)。干预24,48,72 h,采用酶联免疫吸附(ELISA)法测定各组成骨细胞中β-catenin、糖原合成激酶3(GSK-3β)和骨形成蛋白2(BMP2)的表达,采用荧光定量PCR(qPCR)法测定各组成骨细胞中Runt相关转录因子2(Runx2)、成骨细胞特异性转录因子Osterix mRNA的表达情况。结果0~200μmol/L艾司氯胺酮对成骨细胞增殖无明显毒性。与空白组相比,Ket组β-catenin、BMP2以及Runx2和Osterix表达升高(P<0.05),GSK-3β表达降低(P<0.05)。结论艾司氯胺酮可促进小鼠体外成骨细胞增殖和分化,其机制可能与促进β-catenin、BMP2以及Runx2、Osterix表达,降低GSK-3β表达有关。Objective To observe the effect of esketamine on mouse osteoblasts in vitro.Methods Bone marrow derived mesenchymal stem cells(BMSCs)from the hindlimb bones of 4-6-week-old C57BL male mice were selected and differentiated into osteoblasts.The esketamine concentration range with no toxicity to osteoblast proliferation was screened by CCK-8 experiment,and then osteoblasts were randomly divided into two groups:blank group and esketamine group(Ket group,100μmol/L).After 24,48,72 h intervention,the expression levels ofβ-catenin,glycogen synthesis kinase 3(GSK-3β)and bone morphogenetic protein 2(BMP2)in osteoblasts in each group were determined by enzyme-linked immunosorbent assay(ELISA),and the mRNA expression levels of RUNT-associated transcription factor 2(Runx2)and osteoblast-specific transcription factor Osterix were determined by real-time fluorescence quantitative PCR(qPCR).Results Esketamine(0-200μmol/L)had no obvious toxicity to osteoblast proliferation.Compared with blank group,the expression levels ofβ-catenin,BMP2,Runx2 and Osterix were significantly increased in Ket group(P<0.05),while the expression of GSK-3βwas significantly decreased(P<0.05).Conclusion Esketamine can promote the proliferation and the differentiation of mouse osteoblasts in vitro by promoting the expression ofβ-catenin,BMP2,Runx2,Osterix,and decreasing the expression of GSK-3β.

关 键 词：艾司氯胺酮 成骨细胞 -CATENIN GSK-3β BMP2 RUNX2 OSTERIX 

分 类 号：R614[医药卫生—麻醉学]