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作 者:王柏茗 聂江力 裴毅 苏雅 钱滢宇 WANG Baiming;NIE Jiangli;PEI Yi;SU Ya;QIAN Yingyu(College of Horticulture and Landscape of Tianjin Agricultural University,Tianjin 300392,China)
出 处:《云南农业大学学报(自然科学版)》2023年第2期252-261,共10页Journal of Yunnan Agricultural University:Natural Science
基 金:天津市科技计划项目(16PTZSTG00020);天津市津南区国家农业科技园区“一核心三区”专项项目(20YHXSQ005);天津市高等学校大学生创新创业训练计划项目(202010061133,202010061072);天津农学院研究生科研创新项目(2019XY008)。
摘 要:【目的】研究红树莓品种秋萍对盐碱化土壤栽培的适应力,为该品种的建园选址和生产提供参考。【方法】以秋萍红树莓组培苗为材料,盆栽培养一段时间后,用不同浓度的中性盐(NaCl)和碱性盐(NaHCO3)进行单盐胁迫试验,再测定其光合特性以及生理指标。【结果】低浓度(30~60 mmol/L)盐胁迫处理对秋萍生长有促进作用,主要表现为净光合速率和气孔导度增大、色素含量增加、落叶减少以及SOD和POD活性降低;中等浓度(90~120 mmol/L)盐胁迫下,秋萍植株虽然能生长,但呈现生理干旱、光合受到抑制、气孔导度减小、CAT活性升高和脯氨酸含量增加等现象;高浓度(150~180 mmol/L)胁迫下,秋萍的光合受到强烈抑制,叶片色素含量降低,保护酶活性降低,渗透调节物质含量减少,180 mmol/L NaCl胁迫下植株死亡。【结论】秋萍对NaHCO3的耐性较NaCl强,能够适应较大浓度范围的盐碱环境,且轻度盐碱胁迫可促进秋萍生长。[Purposes]To study the adaptability of Rubus idaeus L.‘Qiuping’to stress conditions in saline-alkaline soil cultivation,so as to provide theoretical reference for orchard site selection and production.[Methods]The tissue culture seedlings of Qiuping were used as materials,and after pot culture for a period of time,the single salt stress test was carried out with neutral salt(NaCl)and alkaline salt(NaHCO3).Photosynthetic characteristics and physiological indexes were determined.[Results]Low concentration(30-60 mmol/L)stress could promote the growth of Qiuping,mainly including the increase of net photosynthetic rate and stomatal conductance,the increase of pigment content,the decrease of leaf litter,and the decrease of SOD and POD activities.Under the medium concentration(90-120 mmol/L)stress,Qiuping could grow,but the photosynthesis was inhibited,showing physiological drought,stomatal conductance was decreased,CAT activity was increased,proline content was increased.Under the high concentration(150-180 mmol/L)stress,photosynthesis was strongly inhibited,leaf pigment was decreased,protective enzyme activity was decreased,osmotic regulatory substances were also decreased,and Qiuping died at 180 mmol/L NaCl.[Conclusion]The NaHCO3 tolerance of Qiuping is stronger than that of NaCl,and it can adapt to saline-alkali environment with a large concentration range.Mild saline-alkali stress can promote the growth of Qiuping.
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