阿胶治疗地中海贫血孕妇疗效及对热休克蛋白70表达的影响  

Efficacy of Asini Cori Colla therapy on pregnant women with thalassemia and its effects on the expression of HSP70

作　　者：李艳芳[1] 王淑平[1] 周静文 张战锋[1] 李道成[1] 罗颂平[2] LI Yan-fang;WANG Shu-ping;ZHOU Jing-wen;ZHANG Zhan-feng;LIDao-cheng;LUO Song-ping(First Affliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学,广州510405

出　　处：《中华中医药杂志》2023年第4期1887-1892,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81704111);广东省科技计划项目(No.2017ZC0150)。

摘　　要：目的:观察口服中药阿胶治疗地中海贫血患者妊娠期贫血的疗效以及对热休克蛋白70(HSP70)表达的影响。方法:采用转录组测序方法,检测阿胶治疗前后地中海贫血孕妇基因表达的变化,分析潜在作用靶标。再通过实施一项随机对照研究,验证转录组测序结果。结果:转录组测序分析显示,“蛋白质异二聚活性”“热休克蛋白70结合”以及“相同的蛋白结合”等是富集的差异表达基因的最显著分子功能。口服阿胶治疗4周后,治疗组孕妇外周血血红蛋白(Hb)浓度、HSP70mRNA和蛋白水平均显著高于对照组(P<0.05,P<0.01);且治疗前后Hb浓度差值与HSP70mRNA和蛋白表达量差值存在显著正相关关系(P<0.01)。结论:口服阿胶治疗能有效改善部分地中海贫血孕妇贫血,同时上调HSP70表达,推测阿胶的消化产物,如多肽和氨基酸等,可能作为底物与HSP70等分子伴侣结合,通过蛋白-蛋白相互作用发挥抗贫血作用。Objective:To observe the effects of Asini Cori Colla on anemia and heat shock protein 70(HSP70)level in pregnant women with thalassemia.Methods:Transcriptome sequencing was used to detect the changes of gene expression in pregnant women with thalassemia before and after Asini Cori Colla treatment,and to analyze the potential targets.A randomized controlled study was conducted to confirm the results of transcriptome sequencing.Results:The top molecular functions of differentially expressed genes were mainly involved in the‘Protein Heterodimerization Activity',HSP70 Protein Binding'and ‘Homologous Protein Binding'.After treatment,the Hb concentration,and the level of HSP70 mRNA and HSP70 protein of pregnant women in the treatment group were significantly higher than those in the control group(P<0.05,P<0.01).Moreover,there was a significant positive correlation between the change of Hb concentration and the change of HSP70 mRNA and protein level before and after treatment(P<0.01).Conclusion:Asini Cori Colla therapy can effectively improve anemia in pregnant women with thelassemia.It is speculated that the main digestive produce of Asini Cori Colla,such as polypeptides and amino acids,might occur protein-protein interaction by binding with HSP70 and other molecules,and play an anti-anemiarole.

关 键 词：地中海贫血 贫血 妊娠 阿胶 热休克蛋白70 分子伴侣 疗效 转录组测序 

分 类 号：R714.254[医药卫生—妇产科学]