The U-box ubiquitin ligase TUD1 promotes brassinosteroid-induced GSK2 degradation in rice  

作　　者：Dapu Liu Xiaoxing Zhang Qingliang Li Yunhua Xiao Guoxia Zhang Wenchao Yin Mei Niu Wenjing Meng Nana Dong Jihong Liu Yanzhao Yang Qi Xie Chengcai Chu Hongning Tong 

机构地区：[1]National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China [3]National Nanfan Research Institute,Chinese Academy of Agricultural Sciences,Sanya 572024,China

出　　处：《Plant Communications》2023年第2期107-118,共12页植物通讯（英文）

基　　金：supported by the Hainan Yazhou Bay Seed Laboratory(B21HJ0215);the National Natural Science Foundation of China(nos.U21A20208,31900177,31901534,31871587);the Central Publicinterest Scientific Institution Basal Research Fund(no.S2022ZD02);D.L.was funded by the China Postdoctoral Science Foundation(2020M670548).

摘　　要：Brassinosteroids(BRs)are a class of steroid hormones with great potential for use in crop improvement.De-repression is usually one of the key events in hormone signaling.However,how the stability of GSK2,the central negative regulator of BR signaling in rice(Oryza sativa),is regulated by BRs remains elusive.Here,we identify the U-box ubiquitin ligase TUD1 as a GSK2-interacting protein by yeast two-hybrid screening.We show that TUD1 is able to directly interact with GSK2 and ubiquitinate the protein.Phenotypes of the tud1 mutant are highly similar to those of plants with constitutively activated GSK2.Consistent with this finding,GSK2 protein accumulates in the tud1 mutant compared with the wild type.In addition,inhibition of BR synthesis promotes GSK2 accumulation and suppresses TUD1 stability.By contrast,BRs can induce GSK2 degradation but promote TUD1 accumulation.Furthermore,the GSK2 degradation process is largely impaired in tud1 in response to BR.In conclusion,our study demonstrates the role of TUD1 in BR-induced GSK2 degradation,thereby advancing our understanding of a critical step in the BR signaling pathway of rice.

关 键 词：BRASSINOSTEROID TUD1 GSK2 signal transduction UBIQUITINATION 

分 类 号：Q94[生物学—植物学]