A gap-free genome assembly of Chlamydomonas reinhardtii anddetection of translocations induced by CRISPR-mediated mutagenesis  

作　　者：Zachary L.Payne Gervette M.Penny Tychele N.Turner Susan K.Dutcher 

机构地区：[1]Department of Genetics,Washington University School of Medicine,Saint Louis,MO 63110,USA

出　　处：《Plant Communications》2023年第2期249-271,共23页植物通讯（英文）

基　　金：This work was supported by a grant(R35GM131909)to S.K.D.;a grant(R00MH117165)to T.N.T.G.M.P.was supported by The Bayer Excellence Fund for Graduate Fellowships in Life Sciences.

摘　　要：Genomic assemblies of the unicellular green alga Chlamydomonas reinhardtii have provided important resources for researchers.However,assembly errors,large gaps,and unplaced scaffolds as well as strain-specific variants currently impede many types of analysis.By combining PacBio HiFi and Oxford Nanopore long-read technologies,we generated a de novo genome assembly for strain CC-5816,derived from crosses of strains CC-125 and CC-124.Multiple methods of evaluating genome completeness and base-pair error rate suggest that the final telomere-to-telomere assembly is highly accurate.The CC-5816 assembly enabled previously difficult analyses that include characterization of the 17 centromeres,rDNA arrays on three chromosomes,and 56 insertions of organellar DNA into the nuclear genome.Using Nanopore sequencing,we identified sites of cytosine(CpG)methylation,which are enriched at centromeres.We analyzed CRISPR-Cas9 insertional mutants in the PF23 gene.Two of the three alleles produced progeny that displayed patterns of meiotic inviability that suggested the presence of a chromosomal aberration.Mapping Nanopore reads from pf23-2 and pf23-3 onto the CC-5816 genome showed that these two strains each carry a translocation that was initiated at the PF23 gene locus on chromosome 11 and joined with chromosomes 5 or 3,respectively.The translocations were verified by demonstrating linkage between loci on the two translocated chromosomes in meiotic progeny.The three pf23 alleles display the expected short-cilia phenotype,and immunoblotting showed that pf23-2 lacks the PF23 protein.Our CC-5816 genome assembly will undoubtedly provide an important tool for the Chlamydomonas research community.

关 键 词：ALGA reference genome de novo PacBio NANOPORE structural variation 

分 类 号：Q94[生物学—植物学]