桑树木屑栽培的瓦尼桑黄子实体分级醇沉粗多糖组成特征与活性相关性  

Correlation between composition characteristics and activities of graded ethanol precipitation derived crude polysaccharides of Sanghuangporus vaninii basidiomata cultivated on artificial substrate prepared from mulberry sawdust

作　　者：楚文琪 吴迪[2] 陈万超[2] 李正鹏[2] 张忠[2] 杨焱[2] CHU Wenqi;WU Di;CHEN Wanchao;LI Zhengpeng;ZHANG Zhong;YANG Yan(School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093 [2]上海市农业科学院食用菌研究所、国家食用菌工程技术研究中心、国家食用菌加工技术研发中心、农业农村部南方食用菌资源利用重点实验室,上海201403

出　　处：《菌物学报》2023年第4期984-996,共13页Mycosystema

基　　金：上海市科委农业领域项目(22N51900100)。

摘　　要：对桑树木屑代料栽培瓦尼桑黄Sanghuangporus vaninii子实体分级醇沉粗多糖组分的多糖含量、总酚含量、重均分子量分布、红外光谱、单糖组成、抗氧化活性和免疫活性进行了研究,并采用Pearson相关性分析对各醇沉粗多糖组分的组成特征与活性进行了关联性分析。结果表明,不同醇沉组分粗多糖的组成特征及活性差异较大,其中,各分级醇沉粗多糖组分的多糖含量随着醇沉浓度的增加而增加;20%醇沉多糖组分的总酚含量较高,50%醇沉多糖组分中的总酚含量较低;粗多糖重均分子量随着醇沉浓度的增加而降低;红外光谱结果表明,70%醇沉组分的多糖羟基振动峰不显著,与其分子量分布较低相关;就单糖组成而言,在50%醇沉组分中,果糖及甘露糖含量较高,而在20%和70%醇沉组分中含量较高的单糖是葡萄糖。相关性分析结果显示,子实体粗多糖组分中的总酚含量与DPPH及ABTS自由基清除能力呈显著正相关关系;3个粗多糖组分均能激活RAW 264.7细胞释放NO,促进其分泌肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)和白细胞介素-1β(interleukin-1β,IL-1β)等免疫调节因子,NO释放量与多糖含量呈显著正相关关系,总酚含量较低,且半乳糖和果糖含量较高的S50具有较好的体外免疫活性。本研究为代料栽培瓦尼桑黄子实体多糖类功效成分的研究及开发利用提供参考。The polysaccharide content,total phenol content,weight average molecular weight distribution,Fourier transform infrared spectroscopy,monosaccharide composition,antioxidant activities and immune activities in vitro of graded ethanol precipitation derived crude polysaccharides of Sanghuangporus vaninii fruiting bodies were compared.Pearson correlation analysis was used to analyze the compositional characteristics and activities of graded ethanol precipitation derived crude polysaccharides.The results showed that the physicochemical properties and activities of different ethanol components derived crude polysaccharides were quite different.The polysaccharide content of graded ethanol precipitation derived crude polysaccharides increased with the increase of ethanol precipitation concentration.The content of total phenol was higher in 20%ethanol gradients,and lower in 50%ethanol gradients.The weight average molecular weight decreased with the increase of ethanol precipitation concentration.The infrared spectrum showed that the hydroxyl vibrational peak of the polysaccharide in the 70%alcoholic fraction was not significant and correlated with low molecular weight distribution of the polysaccharide.In terms of monosaccharide composition,fructose and mannose contents were higher in 50%ethanol gradients,while glucose content was higher in 20%and 70%ethanol gradients.Correlation analysis results show that total phenolic content in crude polysaccharides of the basidiomata showed a significant positive correlation with DPPH and ABTS free radical scavenging activity.All the three crude polysaccharide fractions could activate NO release from RAW 264.7 cells and promote their secretion of immunomodulatory factors such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β).There was a significant positive correlation between NO release and polysaccharide content,and S50 with lower total phenolic content and higher galactose and fructose content had better immune activity in vitro.This study provides

关 键 词：桑黄多糖 分级醇沉 抗氧化 体外免疫活性 相关性分析 

分 类 号：R284.1[医药卫生—中药学]