转基因大豆对建鲤抗氧化、生化及免疫指标的影响  

Effects of Glyphosate-Resistant Genetically Modified Soybean on Antioxidant Capacity,Biochemical and Immune Indices of Jian Carp Cyprinus carpio var.jian

作　　者：俞涛涛 张家国 黄小霞[1,2] 华茂圳 蒋乐霞 黄宝生 张长峰[2,3] YU Taotao;ZHANG Jiaguo;HUANG Xiaoxia;HUA Maozhen;JIANG Lexia;HUANG Baosheng;ZHANG Changfeng(Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN),Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Shandong Vocational and Technical College of Commerce,Jinan 250103,China;Shandong Provincial Key Laboratory of Storage and Transportation Technology for Agricultural Products,Jinan 250103,China;Guangdong Ocean University,Zhanjiang 524000,China)

机构地区：[1]上海海洋大学,农业农村部鱼类营养与环境生态研究中心,上海201306 [2]山东商业职业技术学院,山东济南250103 [3]山东省农产品贮运保鲜技术重点实验室,山东济南250103 [4]广东海洋大学,广东湛江524000

出　　处：《水产科学》2023年第3期357-366,共10页Fisheries Science

基　　金：农业农村部淡水渔业与种质资源利用重点实验室开放基金课题项目(XKQKF201901);2019年山东省农业重大应用技术创新项目(SD2019YY005);泰山学者特聘专家项目(TS20190956).

摘　　要：为评价抗草甘膦转基因大豆对建鲤的饲用安全性,试验以480尾平均体质量(60±10)g的建鲤幼鲤为对象,随机分为4组,分别添加15%和30%经过高温预处理的抗草甘膦转基因大豆制成的试验饲料,以同等添加比例的高温预处理非转基因大豆饲料作为对照,饲养周期为180d,采用常规方法测定生长指标,采用可见光法、干粉法等测定相关肝胰脏与血液指标。试验结果表明:饲料中添加15%和30%抗草甘膦转基因大豆对建鲤的生长性能、脏器指数和肌肉常规营养组分均未见显著影响(P>0.05),建鲤肝胰脏谷胱甘肽过氧化物酶活性显著升高(P<0.05);饲料中添加30%的抗草甘膦转基因大豆会显著降低肝胰脏过氧化氢酶活性(P<0.05),显著升高丙二醛水平(P<0.05),显著降低建鲤血清中碱性磷酸酶活性(P<0.05)。综上,饲料中添加高水平的抗草甘膦转基因大豆会对建鲤的碱性磷酸酶活性产生一定程度的负面影响,而影响建鲤肝胰脏抗氧化能力的根本原因是否为转基因这一因素还需进一步探究。In order to evaluate the safety of glyphosate-resistant genetically modified soybeans(GMS)for Jian carp Cyprinus carpio var.jian,480 juveniles with average body weight of(60±10)g were used as the test subjects in the experiment and randomly divided into four groups.The test diet was supplemented with 15%and 30%glyphosate-resistant GMS pretreated with high temperature,respectively,and the same proportion of non-GMS diet pretreated with high temperature was used as control.The feeding period was 180 d.Growth indicators were determined by conventional methods,and related hepatopancreas and blood indicators were determined by visible light method and dry powder method.The results showed that the addition of 15%and 30%glyphosate-resistant GMS to the diet did not led to significant effect on the growth index,organ index,and muscle routine nutritional components of carp(P>0.05).The activity of GSH-Px in hepatopancreas was significantly increased when the amount of GMS in the diet was 15%and 30%(P<0.05).The activity of CAT in hepatopancreas was significantly reduced and the level of MDA in hepatopancreatic was significantly increased by adding 30%GMS(P<0.05).The ALP activity in carp serum was significantly reduced by 30%in the fish fed the diet containing 30%of GMS(P<0.05).Therefore,adding a high amount of glyphosate-resistant GMS to the diet will significantly affect the antioxidant capacity of the hepatopancreatic of Jian carp,and have a certain degree of negative impact on the immunity of the carp.However,whether the critical cause of these effects is genetically modified or not needs to be further explored.

关 键 词：抗草甘膦转基因大豆 建鲤 生长性能 抗氧化能力 免疫 

分 类 号：S965.116[农业科学—水产养殖]