细胞色素P450基因家族参与植物硫代葡萄糖苷生物合成的研究进展  

Research Progress of Cytochrome P450 Gene Family Taking Part in Biosynthesis of Plant Glucosinolates

作　　者：费丹丹 王军伟 吴小媚 黄惠萍 毛舒香 吴秋云[1] 黄科[1] FEI Dandan;WANG Junwei;WU Xiaomei;HUANG Huiping;MAO Shuxiang;WU Qiuyun;HUANG Ke(College of Horticulture,Hunan Agricultural University,Key Laboratory for Vegetable Biology of Hunan Province,Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education,Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops(Vegetables,Tea,etc.),Ministry of Agriculture and Rural Affairs of China,Changsha 410128,Hunan,China)

机构地区：[1]湖南农业大学园艺学院,蔬菜生物学湖南省重点实验室,园艺作物种质创新与新品种选育教育部工程研究中心,农业农村部园艺作物(蔬菜、茶叶等)基因资源评价利用重点实验室,湖南长沙410128

出　　处：《中国蔬菜》2023年第5期30-41,共12页China Vegetables

基　　金：国家自然科学基金项目(32072568);湖南省自然科学基金项目(2021JJ30345,2021JJ30324,2022JJ30297)。

摘　　要：硫代葡萄糖苷是一种主要存在于十字花科植物中的重要次生代谢物,在植物病虫害防御、食品风味形成以及医学癌症治疗等方面均有重要作用。硫代葡萄糖苷的生物合成过程十分复杂,参与其合成调控的因素有很多,如遗传背景、调节基因以及植物激素等。细胞色素P450酶(CYP450)是一类普遍存在于动植物、微生物以及病毒中的超基因家族编码的酶系,在硫代葡萄糖苷的生物合成中起关键作用。本文综述了CYP450的结构、分类和功能,阐述了CYP450参与硫代葡萄糖苷生物合成的作用,并介绍了CYP450响应外源刺激和环境条件的变化调控硫代葡萄糖苷合成的作用机理,以期为研究硫代葡萄糖苷合成机制提供参考。Glucosinolate is an important secondary metabolite mainly presented in cruciferous plants.It plays an important role in plant disease and insect defense,food flavor formation,and cancer medical treatment,etc.The biosynthetic process of glucosinolates is very complex.There are many factors involved in its synthesis regulation,such as genetic background,regulatory genes and plant hormones,etc.Cytochrome P450 is a family of enzyme lines encoded by supergenes that are ubiquitous in animals,plants,microorganisms and viruses.It plays a key role in the biosynthesis of glucosinolates.This paper reviewed the structure,classification and function of cytochrome P450,expounded the role of cytochrome P450 in glucosinolate biosynthesis,and introduced the mechanism of cytochrome P450 in regulating glucosinolate synthesis responsing to changes of exogenous stimulation and environmental conditions,hoping to provide reference for studying the mechanism of glucosinolate synthesis.

关 键 词：细胞色素P450 硫代葡萄糖苷 生物合成 外源刺激 环境条件 

分 类 号：Q943.2[生物学—植物学]