甜瓜SAUR基因家族鉴定及生物信息学分析  

Identification and Bioinformatics Analysis of SAUR Gene Family in Melon(Cucumis melo L.)

作　　者：焦莉苹 司修洋 郭子雯 李鹏飞[1] 刘大伟[1] JIAO Liping;SI Xiuyang;GUO Ziwen;LI Pengfei;LIU Dawei(College of Plant Protection,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang,China)

机构地区：[1]东北农业大学植物保护学院,黑龙江哈尔滨150030 [2]浙江大学农业与生物技术学院,浙江杭州310058

出　　处：《中国蔬菜》2023年第5期42-55,共14页China Vegetables

基　　金：黑龙江省博士后科研启动金资助项目(LBH-Q19081)。

摘　　要：SAUR(smallauxin-upRNA)为早期生长素应答重要基因家族之一。采用生物信息学方法,对甜瓜SAUR家族基因进行系统地鉴定,并对其基因结构、进化关系、基因复制关系、基因组织表达水平等进行分析。结果表明:在甜瓜基因组中共鉴定出66个SAUR基因,在1号、11号染色体上分布的基因数目较多。通过最大似然法对甜瓜、拟南芥、西瓜、黄瓜SAUR家族基因蛋白氨基酸全长序列构建系统进化树,划分成5个类群,甜瓜与拟南芥的SAUR基因在进化树上更倾向于分布在不同的分支上,而与西瓜、黄瓜的SAUR基因在进化树上更倾向于聚在一起。共鉴定到6个串联重复事件以及11个片段复制基因对;甜瓜SAUR家族基因中有13个基因在6个物种中均可形成基因对。顺式作用元件预测鉴定到8个与非生物和生物胁迫相关的顺式作用元件,10个与植物激素响应相关的顺式作用元件,8个与植物生长发育相关的顺式作用元件。甜瓜SAUR家族基因在不同组织中的表达水平差异较大,在甜瓜不同发育阶段表达亦有所不同。Small auxin-up RNA(SAUR)gene family is the most important family of early auxin response genes in higher plants.We identified the SAUR family genes in melon,analyzed the gene structure,evolutionary relationship,gene duplication relationship,and gene tissue expression level using bioinformatics methods.The results showed that a total of 66 SAUR genes were identified in the melon genome,and the number of SAUR genes distributed on chromosomes 1 and 11 was much higher.Phylogenetic trees were constructed based on the full-length amino acid sequences of SAUR family genes in melon,Arabidopsis,watermelon,and cucumber by the maximum likelihood method,and SAUR genes can be classified into 5 groups.The SAUR genes of melon and Arabidopsis tended to be distributed in different branches on the evolutionary tree,while the SAUR genes of melon,watermelon and cucumber tended to cluster together.A total of 6 tandem duplication events and 11 segmental duplication gene pairs were identified.In addition,13 genes could form gene pairs in all six species.Eight cis-acting elements were related to abiotic and biotic stress responses,10 cis-acting elements were related to plant hormone responses,and 8 cis-acting elements were related to plant growth and development.The expression levels of melon SAUR family genes were different in different tissues and developmental stages of melon.

关 键 词：甜瓜 SAUR基因家族 系统进化树 基因复制关系 表达模式 

分 类 号：S652[农业科学—果树学]