壮药斜叶黄檀中1对新的异黄烷类对映异构体  

A pair of new isoflavan enantiomers from Zhuang medicine Dalbergia pinnata

作　　者：王维玉 何欢 朱敏 宁小燕 覃川娴 卢澄生 刘锴[4] 冯旭 韦建华 WANG Wei-yu;HE Huan;ZHU Min;NING Xiao-yan;QIN Chuan-xian;LU Cheng-sheng;LIU Kai;FENG Xu;WEI Jian-hua(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Nanning 530200,China;National Demonstration Center for Experimental Chinese Medicine Education,Guangxi University of Chinese Medicine,Nanning 530200,China;Institute of Marine Drugs,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西壮瑶药重点实验室,广西南宁530200 [3]广西中医药大学中药学国家级实验教学示范中心,广西南宁530200 [4]广西中医药大学海洋药物研究,广西南宁530200

出　　处：《中草药》2023年第7期2021-2027,共7页Chinese Traditional and Herbal Drugs

基　　金：广西壮瑶药重点实验室项目(桂科基字[2014]32号);广西壮瑶药重点实验室开放课题(GXZY22.2019-10);广西一流学科建设开放课题自然科学项目(2019XK129);广西中医药大学2021年研究生教育创新计划项目立项课题(YCXJ2021018);广西中医药大学2021大学生创新创业训练计划项目(202110600160);广西中医药大学自然科学基金项目(2022MS031)。

摘　　要：目的研究壮药斜叶黄檀Dalbergia pinnata根茎的化学成分。方法通过硅胶柱色谱、RP-C18及半制备高效液相色谱法进行分离和纯化,根据理化性质、波谱数据及结合参考文献鉴定化合物结构并对分离得到的化合物进行体外抗氧化活性测试。结果从斜叶黄檀乙醇提取物中分离得到13个化合物,分别鉴定为斜叶黄檀素A(1a)、斜叶黄檀素B(1b)、2’-甲氧基-4’-羟基-α,β-二氢查耳酮(2)、3,7-二羟基-6-甲氧基-二氢黄酮(3)、8-羟基-4’,7-二甲氧基异黄酮(4)、黑黄檀素(5)、黄檀素(6)、6-羟基-7-甲氧基-二氢黄酮(7)、赝靛黄素(8)、8-O-methylretusin(9)、二氢芒柄花黄素(10)、2’-甲氧基-4’-羟基查耳酮(11)、5,7,3’-三羟基-5’-甲氧基-异黄酮(12)。结论化合物1a和1b为1对新的异黄烷对映异构体,化合物7为新的天然产物,所有化合物均为首次从斜叶黄檀植物中分离得到。化合物1(1a+1b)、3~4、6~7、9~12表现出较好的体外抗氧化能力,与阳性对照维生素C相当。Objective To study the chemical constituents of Zhuang medicine Dalbergia pinnata.Methods The chemical constituents were isolated and purified by silica gel,RP-C18 and semi-preparative high performance liquid chromatography.Their structures were identified by physicochemical properties,spectral data as well as relevant references.The isolated compounds were tested for antioxidant activity in vitro.Results Thirteen compounds were isolated from the ethanol fraction of D.pinnata and identified as dalbergipinnatamin A(1a),dalbergipinnatamin B(1b),2'-methoxy-4'-hydroxy-α,β-dihydrochalcone(2),3,7-dihydroxy-6-methoxy-dihydroflavone(3),8-hydroxy-4',7-dimethoxyisoflavone(4),caviunin(5),dalbergin(6),6-hydroxy-7-methoxy-dihydroflavone(7),pseudobaptigenin(8),8-O-methylreusin(9),dihydroformononetin(10),2'-methoxy-4'-hydroxychalcone(11),5,7,3'-trihydroxy-5'-methoxy-isoflavone(12).Conclusion Compounds 1 and 2 is a pair of new isoflavan enantiomers,compound 7 is a new natural product.All compounds were isolated from D.pinnata for the first time.Compounds 1,3-4,6-7,9-12 showed good in vitro antioxidant capacity,which was comparable to the positive control vitamin C.

关 键 词：斜叶黄檀 黄酮类 斜叶黄檀素 抗氧化活性 斜叶黄檀素A 斜叶黄檀素B 3 7-二羟基-6-甲氧基-二氢黄酮 黄檀素 二氢芒柄花黄素 

分 类 号：R284.1[医药卫生—中药学]