基于纳米金类过氧化物酶活性构建RGB可视化检测体系的研究  

Study on the construction of RGB visual detection system based on the peroxidase-like activity of gold nanoparticles

作　　者：吴硕 罗圆圆 王永辉 王美玲 高志贤 周焕英 WU Shuo;LUO Yuan-Yuan;WANG Yong-Hui;WANG Mei-Ling;GAO Zhi-Xian;ZHOU Huan-Ying(Institute of Environmental and Operational Medicine,Academy of Military Medical Science,Academy of Military Science,Tianjin Key Laboratory of Environmental and Food Safety Risk Monitoring Technology,Tianjin 300050,China)

机构地区：[1]军事科学院军事医学研究院环境医学与作业医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050

出　　处：《食品安全质量检测学报》2023年第6期54-60,共7页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2017YFC1601101)。

摘　　要：目的利用金纳米粒子(goldnanoparticles,AuNPs)的类过氧化物酶催化活性,结合智能手机RGB识别进行可视化信号采集,构建颜色-RGB数值-模拟曲线响应模型。方法采用柠檬酸钠还原法合成粒径均一的AuNPs分散液。利用一级动力学模型对AuNPs催化活性进行测定并探究不同反应条件对催化活性的影响。借助智能手机软件Color Meter获得反应后图像的RGB参数信息,选择最优定量公式拟合实验数据。结果AuNPs表观催化活性为3.5×10^(-3)s^(-1)·mL^(-1)。反应条件在40~50℃、pH 3.5~4.0、醋酸钠缓冲液浓度大于1.0 mmol/L、反应时间大于15 min时AuNPs催化活性最高,AuNPs的量与反应后溶液显色程度在一定范围内呈现良好的线性关系。结论AuNPs可通过控制反应参数呈现不同的类过氧化物酶催化活性,利用智能手机RGB识别技术可快速、直观地展现不同量AuNPs催化显色反应的结果,完成定量分析。该方法具有良好的准确度和可靠性,可作为一种可视化检测手段,为利用AuNPs构建的生物传感检测体系提供参考。Objective To construct a color-RGB numeric-simulation curve response model by utilizing the peroxidase-like activity of gold nanoparticles(AuNPs)combined with the RGB recognition function of smart phone for visual signal acquisition.Methods AuNPs disperse solution with uniform particle size were synthesized by sodium citrate reduction method.The first order kinetic model was used to determine the catalytic activity of AuNPs and explore the effects of different reaction conditions on the catalytic activity.Color Meter,a smart phone software,was used to obtain the RGB parameter information of the image after reaction,and the optimal quantitative formula was selected to fit the experimental data.Results The apparent catalytic activity of AuNPs was 3.5×10^(‒3)s^(‒1)·mL^(‒1).The highest catalytic activity of AuNPs was observed at 40‒50℃,pH 3.5‒4.0,sodium acetate buffer concentration more than 1.0 mmol/L,and reaction time more than 15 min.The AuNPs content had a good linear relationship with the color change degree of solution after reaction.Conclusion AuNPs can present different peroxidase-like catalytic activities by controlling the reaction parameters,the results of different amounts of AuNPs catalyzed color reaction can be quickly and intuitively displayed by using the RGB recognition technology of smart phone,and the quantitative analysis is completed.This method has good accuracy and reliability,and can be used as a visual detection method to provide a reference for the biosensing detection system constructed by AuNPs.

关 键 词：纳米金 过氧化物酶 RGB比色法 可视化检测 

分 类 号：TB383.1[一般工业技术—材料科学与工程] O657.3[理学—分析化学] TS207.3[理学—化学]